Effects of downstream genes on synthetic genetic circuits

Takefumi Moriya, Masayuki Yamamura, Daisuke Kiga*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Background: In order to understand and regulate complex genetic networks in living cells, it is important to build simple and well-defined genetic circuits. We designed such circuits using a synthetic biology approach that included mathematical modeling and simulation, with a focus on the effects by which downstream reporter genes are involved in the regulation of synthetic genetic circuits. Results: Our results indicated that downstream genes exert two main effects on genes involved in the regulation of synthetic genetic circuits: (1) competition for regulatory proteins and (2) protein degradation in the cell. Conclusions: Our findings regarding the effects of downstream genes on regulatory genes and the role of impedance in driving large-scale and complex genetic circuits may facilitate the design of more accurate genetic circuits. This design will have wide applications in future studies of systems and synthetic biology.

Original languageEnglish
Article numberS4
JournalBMC Systems Biology
Issue number4
Publication statusPublished - 2014 Dec 8
Externally publishedYes


  • Decoy site
  • Genetic circuit
  • Impedance
  • Mathematical modeling
  • Protein degradation
  • Synthetic biology

ASJC Scopus subject areas

  • Structural Biology
  • Modelling and Simulation
  • Molecular Biology
  • Computer Science Applications
  • Applied Mathematics


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