Effects of hindlimb unloading on neurogenesis in the hippocampus of newly weaned rats

Sachiko Nomura, Katsuya Kami, Fuminori Kawano, Yoshihiko Oke, Naoya Nakai, Takashi Ohira, Ryo Fujita, Masahiro Terada, Kazuhiko Imaizumi, Yoshinobu Ohira*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)


    Effects of hindlimb suspension (HS) and ambulation recovery on hippocampal neurogenesis of newly weaned rats were studied by using immunohistochemical techniques. The number of proliferating cell nuclear antigen-positive (PCNA+) cells in the subgranular zone (SGZ) markedly decreased during normal growth. However, neither HS nor subsequent recovery caused additional changes in the number of PCNA+ cells. The number of doublecortin-positive (DCX+) neurons decreased gradually during normal growth. HS resulted in a further decrease in these neurons. However, DCX+ cell numbers became identical to the levels in age-matched controls after 14 days of recovery. PCNA and DCX-double positive cells in the SGZ were also observed, and their cell numbers were not affected by HS and 14-day ambulation. Thus, HS suppressed the generation of DCX+ neurons without affecting PCNA+ cells in the SGZ of weaned rats. Taken together, hippocampal neurogenesis in weaned rats was not severely affected by HS while it decreased significantly as they had grown.

    Original languageEnglish
    Pages (from-to)76-81
    Number of pages6
    JournalNeuroscience Letters
    Issue number2
    Publication statusPublished - 2012 Feb 16


    • Doublecortin-positive neurons
    • Hindlimb suspension
    • Hippocampal neurogenesis
    • Newly weaned rat

    ASJC Scopus subject areas

    • Neuroscience(all)


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