TY - JOUR
T1 - Effects of hindlimb unloading on neurogenesis in the hippocampus of newly weaned rats
AU - Nomura, Sachiko
AU - Kami, Katsuya
AU - Kawano, Fuminori
AU - Oke, Yoshihiko
AU - Nakai, Naoya
AU - Ohira, Takashi
AU - Fujita, Ryo
AU - Terada, Masahiro
AU - Imaizumi, Kazuhiko
AU - Ohira, Yoshinobu
PY - 2012/2/16
Y1 - 2012/2/16
N2 - Effects of hindlimb suspension (HS) and ambulation recovery on hippocampal neurogenesis of newly weaned rats were studied by using immunohistochemical techniques. The number of proliferating cell nuclear antigen-positive (PCNA+) cells in the subgranular zone (SGZ) markedly decreased during normal growth. However, neither HS nor subsequent recovery caused additional changes in the number of PCNA+ cells. The number of doublecortin-positive (DCX+) neurons decreased gradually during normal growth. HS resulted in a further decrease in these neurons. However, DCX+ cell numbers became identical to the levels in age-matched controls after 14 days of recovery. PCNA and DCX-double positive cells in the SGZ were also observed, and their cell numbers were not affected by HS and 14-day ambulation. Thus, HS suppressed the generation of DCX+ neurons without affecting PCNA+ cells in the SGZ of weaned rats. Taken together, hippocampal neurogenesis in weaned rats was not severely affected by HS while it decreased significantly as they had grown.
AB - Effects of hindlimb suspension (HS) and ambulation recovery on hippocampal neurogenesis of newly weaned rats were studied by using immunohistochemical techniques. The number of proliferating cell nuclear antigen-positive (PCNA+) cells in the subgranular zone (SGZ) markedly decreased during normal growth. However, neither HS nor subsequent recovery caused additional changes in the number of PCNA+ cells. The number of doublecortin-positive (DCX+) neurons decreased gradually during normal growth. HS resulted in a further decrease in these neurons. However, DCX+ cell numbers became identical to the levels in age-matched controls after 14 days of recovery. PCNA and DCX-double positive cells in the SGZ were also observed, and their cell numbers were not affected by HS and 14-day ambulation. Thus, HS suppressed the generation of DCX+ neurons without affecting PCNA+ cells in the SGZ of weaned rats. Taken together, hippocampal neurogenesis in weaned rats was not severely affected by HS while it decreased significantly as they had grown.
KW - Doublecortin-positive neurons
KW - Hindlimb suspension
KW - Hippocampal neurogenesis
KW - Newly weaned rat
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U2 - 10.1016/j.neulet.2011.12.022
DO - 10.1016/j.neulet.2011.12.022
M3 - Article
C2 - 22206837
AN - SCOPUS:84856400109
SN - 0304-3940
VL - 509
SP - 76
EP - 81
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 2
ER -