TY - JOUR
T1 - Effects of photodynamic therapy with talaporfin sodium on squamous cell carcinoma and sarcoma cells
AU - Osaki, Tomohiro
AU - Kawase, Yuki
AU - Iseki, Hiroshi
AU - Kishimoto, Shinji
AU - Ikuta, Soko
AU - Muragaki, Yoshihiro
AU - Yamashita, Masamichi
AU - Azuma, Kazuo
AU - Murahata, Yusuke
AU - Tsuka, Takeshi
AU - Itoh, Norihiko
AU - Imagawa, Tomohiro
AU - Okamoto, Yoshiharu
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - Background Photodynamic therapy (PDT) uses a photosensitizer and light to destroy abnormal cells. Talaporfin sodium (NPe6) is a second-generation photosensitizer. Methods We evaluated the toxic effects of different combinations of laser and NPe6 doses on squamous cell carcinoma (KLN205) and sarcoma (Meth A) cell lines. The cells were incubated with 0, 5, 10, or 30 μg/mL NPe6 for 24 h. The cells were then irradiated with 0, 5, 15, or 30 mW/cm2 of laser power, and 0, 1, 5, 10, or 20 J/cm2 of laser energy. Cell viability was evaluated after 24 h. We also evaluated the cytotoxic effects of continuous wave or square-wave modulated laser irradiations (2, 5, or 10 Hz, 50% duty) on Meth A cells. Results The median lethal doses of NPe6 against the KLN205 and Meth A cells after treatment at a fluence rate of 15 mW/cm2 and a light dose of 20 J/cm2 were 18.6 and 5.0 μg/mL, respectively. Meth A cells were more sensitive to PDT than KLN205 cells. There was no significant difference between the effects of continuous wave and square-wave modulated lasers on Meth A cell viability. Conclusions NPe6 PDT induced cell death in a dose-dependent manner in KLN205 and Meth A cells. More work is required to evaluate the cytotoxic effects of square-wave modulated laser therapy at low light doses.
AB - Background Photodynamic therapy (PDT) uses a photosensitizer and light to destroy abnormal cells. Talaporfin sodium (NPe6) is a second-generation photosensitizer. Methods We evaluated the toxic effects of different combinations of laser and NPe6 doses on squamous cell carcinoma (KLN205) and sarcoma (Meth A) cell lines. The cells were incubated with 0, 5, 10, or 30 μg/mL NPe6 for 24 h. The cells were then irradiated with 0, 5, 15, or 30 mW/cm2 of laser power, and 0, 1, 5, 10, or 20 J/cm2 of laser energy. Cell viability was evaluated after 24 h. We also evaluated the cytotoxic effects of continuous wave or square-wave modulated laser irradiations (2, 5, or 10 Hz, 50% duty) on Meth A cells. Results The median lethal doses of NPe6 against the KLN205 and Meth A cells after treatment at a fluence rate of 15 mW/cm2 and a light dose of 20 J/cm2 were 18.6 and 5.0 μg/mL, respectively. Meth A cells were more sensitive to PDT than KLN205 cells. There was no significant difference between the effects of continuous wave and square-wave modulated lasers on Meth A cell viability. Conclusions NPe6 PDT induced cell death in a dose-dependent manner in KLN205 and Meth A cells. More work is required to evaluate the cytotoxic effects of square-wave modulated laser therapy at low light doses.
KW - Continuous wave laser
KW - Photodynamic therapy
KW - Sarcoma
KW - Squamous cell carcinoma
KW - Square-wave modulated laser
KW - Talaporfin sodium
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U2 - 10.1016/j.pdpdt.2017.02.007
DO - 10.1016/j.pdpdt.2017.02.007
M3 - Article
C2 - 28232036
AN - SCOPUS:85016038609
SN - 1572-1000
VL - 18
SP - 213
EP - 220
JO - Photodiagnosis and Photodynamic Therapy
JF - Photodiagnosis and Photodynamic Therapy
ER -