TY - JOUR
T1 - Effects of polyamines on a continuous cell-free protein synthesis system of an extreme thermophile, Thermus thermophilus
AU - Uzawa, Taketoshi
AU - Yamagishi, Akihiko
AU - Ueda, Takuya
AU - Chikazumi, Nobutoshi
AU - Watanabe, Kimitsuna
AU - Oshlma, Tairo
N1 - Funding Information:
This work was partly supported by research funds from the Mitsu-bishi Foundation. The authors also thank Idemitsu Kosan Co. for support.
PY - 1993/11
Y1 - 1993/11
N2 - A continuous cell-free protein synthesis system of an extremely thermophilic eubacterium, Thermus thermophilus HB27, was constructed. This system produced MS2 phage RNA translation products at a rate of more than 5 μg per hour per 1.9 mg of ribosomes at 65°C and the production continued linearly for at least 340 min. When no polyamine was added, the system did not produce the proteins. The highest activity was recorded when 0.1 mM tetrakis(3-aminopropyl)ammonium and 1.0 mM spermine were added simultaneously.
AB - A continuous cell-free protein synthesis system of an extremely thermophilic eubacterium, Thermus thermophilus HB27, was constructed. This system produced MS2 phage RNA translation products at a rate of more than 5 μg per hour per 1.9 mg of ribosomes at 65°C and the production continued linearly for at least 340 min. When no polyamine was added, the system did not produce the proteins. The highest activity was recorded when 0.1 mM tetrakis(3-aminopropyl)ammonium and 1.0 mM spermine were added simultaneously.
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U2 - 10.1093/oxfordjournals.jbchem.a124245
DO - 10.1093/oxfordjournals.jbchem.a124245
M3 - Article
C2 - 8113228
AN - SCOPUS:0027331405
SN - 0021-924X
VL - 114
SP - 732
EP - 734
JO - Journal of biochemistry
JF - Journal of biochemistry
IS - 5
ER -