TY - JOUR
T1 - Effects of the pH-controlled hemoglobin vesicles by CO2 gas
AU - Park, Sungick
AU - Kose, Takehiro
AU - Hamasaki, Masaomi
AU - Takeoka, Shinji
AU - Nishide, Hiroyuki
AU - Tsuchida, Eishun
N1 - Funding Information:
This work was partially supported by the project of Materials Research Laboratory for Bioscience and Photonics in Waseda University, Health Science Research Grant (Artificial Blood Project) from the Ministry of Hearth and Welfare, Japan, Grants-in-Aid from the Ministry of Education, Science, and Culture, Japan (08680940),a nd Kawakami Memorial Foundation.
PY - 1998
Y1 - 1998
N2 - The hemoglobin vesicle (HbV) is a red cell substitute encapsulating purified concentrated Hb in a phospholipid vesicle. In order to improve the oxygen carrying capability of HbV, the pH value of the Hb solution should be adjusted to 7.0 in the HbV preparation, and then the pH value should be adjusted to 7.4 where HbV functions as an oxygen carrier, because the maximum value of [Hb]/[Lipid] was obtained in which the pH of the Hb solution was 7.0, and the metHb formation rate was suppressed in the pH 7.4. Generally, the pH control of the inner aqueous phase of HbV is difficult by changing the pH in the outer phase. We could control the pH of the Hb solution from 7.4 to 7.0 by dissolving CO2 into the Hb solution, and after the preparation of HbV, the pH of HbV is changed to 7.4 by reducing the pressure. The resulting pH-controlled HbV by CO2 gas showed a high [Hb]/[Lipid] value of 1.7 with a low rate of metHb formation.
AB - The hemoglobin vesicle (HbV) is a red cell substitute encapsulating purified concentrated Hb in a phospholipid vesicle. In order to improve the oxygen carrying capability of HbV, the pH value of the Hb solution should be adjusted to 7.0 in the HbV preparation, and then the pH value should be adjusted to 7.4 where HbV functions as an oxygen carrier, because the maximum value of [Hb]/[Lipid] was obtained in which the pH of the Hb solution was 7.0, and the metHb formation rate was suppressed in the pH 7.4. Generally, the pH control of the inner aqueous phase of HbV is difficult by changing the pH in the outer phase. We could control the pH of the Hb solution from 7.4 to 7.0 by dissolving CO2 into the Hb solution, and after the preparation of HbV, the pH of HbV is changed to 7.4 by reducing the pressure. The resulting pH-controlled HbV by CO2 gas showed a high [Hb]/[Lipid] value of 1.7 with a low rate of metHb formation.
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U2 - 10.3109/10731199809117470
DO - 10.3109/10731199809117470
M3 - Article
C2 - 9844716
AN - SCOPUS:0032211609
SN - 2169-1401
VL - 26
SP - 497
EP - 506
JO - Artificial Cells, Nanomedicine and Biotechnology
JF - Artificial Cells, Nanomedicine and Biotechnology
IS - 5-6
ER -