Elastic properties of living fibroblasts as imaged using force modulation mode in atomic force microscopy

Shigeo Sasaki; Mayumi Morimoto; Hisashi Haga; Kazushige Kawabata; Etsuro Ito; Tatsuo Ushiki; Kazuhiro Abe; Takashi Sambongi

doi:10.1679/aohc.61.57

Elastic properties of living fibroblasts as imaged using force modulation mode in atomic force microscopy

Shigeo Sasaki, Mayumi Morimoto, Hisashi Haga^*, Kazushige Kawabata, Etsuro Ito, Tatsuo Ushiki, Kazuhiro Abe, Takashi Sambongi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Using the force modulation mode in atomic force microscopy, we measured elastic properties of living mouse fibroblasts (NIH3T3) in a culture medium. The topographic images of the cellular surface and the corresponding elastic images of the cellular surface were able to be captured simultaneously with high spatial resolution. The consecutive images were useful for examining time-dependent changes in the cellular surface. We observed that some cells continued to shrink and change their softness for 2 hours. Then the force modulation mode in atomic force microscopy shows potential use in analyzing time-dependent regional elastic properties of living cells with high spatial resolution.

Original language	English
Pages (from-to)	57-63
Number of pages	7
Journal	Archives of Histology and Cytology
Volume	61
Issue number	1
DOIs	https://doi.org/10.1679/aohc.61.57
Publication status	Published - 1998 Mar
Externally published	Yes

ASJC Scopus subject areas

Histology

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abstract = "Using the force modulation mode in atomic force microscopy, we measured elastic properties of living mouse fibroblasts (NIH3T3) in a culture medium. The topographic images of the cellular surface and the corresponding elastic images of the cellular surface were able to be captured simultaneously with high spatial resolution. The consecutive images were useful for examining time-dependent changes in the cellular surface. We observed that some cells continued to shrink and change their softness for 2 hours. Then the force modulation mode in atomic force microscopy shows potential use in analyzing time-dependent regional elastic properties of living cells with high spatial resolution.",

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year = "1998",

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AU - Sasaki, Shigeo

AU - Morimoto, Mayumi

AU - Haga, Hisashi

AU - Kawabata, Kazushige

AU - Ito, Etsuro

AU - Ushiki, Tatsuo

AU - Abe, Kazuhiro

AU - Sambongi, Takashi

PY - 1998/3

Y1 - 1998/3

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AB - Using the force modulation mode in atomic force microscopy, we measured elastic properties of living mouse fibroblasts (NIH3T3) in a culture medium. The topographic images of the cellular surface and the corresponding elastic images of the cellular surface were able to be captured simultaneously with high spatial resolution. The consecutive images were useful for examining time-dependent changes in the cellular surface. We observed that some cells continued to shrink and change their softness for 2 hours. Then the force modulation mode in atomic force microscopy shows potential use in analyzing time-dependent regional elastic properties of living cells with high spatial resolution.

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Elastic properties of living fibroblasts as imaged using force modulation mode in atomic force microscopy

Abstract

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