Elastic scattering spectroscopy of coagulated brain tissues

Filiz Ates*, Haşim Özgür Tabakoǧlu, Özgüncem Bozkulak, Murat Canpolat, Murat Gülsoy

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution


The goal of this study was to differentiate the parts of lamb brain according to elastic scattering spectroscopy and detect the optical alterations due to coagulation. Cells and tissues are not uniform and have complex structures and shapes. They can he referred to as scattering particles. The process of scattering depends on the light wavelength and on the scattering medium properties; especially on the size and the density of the medium. When elastic scattering spectroscopy (ESS) is employed, the morphological alterations of tissues can be detected using spectral measurements of the elastic scattered light over a wide range of wavelengths. In this study firstly, the slopes of ESS spectra were used to differentiate the parts of lamb brains (brainstem, cerebellum, gray matter, white matter) in vitro in the range of 450 - 750 nm. Secondly, tissues were coagulated at different temperatures (45, 60, and 80°C and ESS spectra were taken from native and coagulated tissues. It was observed that as the coagulation temperature increased, the slope of the elastic scattering spectra decreased. Thus, optical properties of tissues were changed with respect to the change in nuclear to cytoplasmic ratio due to the water loss. Results showed that the slopes of ESS spectra in the visible range revealed valuable information about the morphological changes caused by coagulation.

Original languageEnglish
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Publication statusPublished - 2006
Externally publishedYes
EventOptical Interactions with Tissue and Cells XVII - San Jose, CA, United States
Duration: 2006 Jan 232006 Jan 25


OtherOptical Interactions with Tissue and Cells XVII
Country/TerritoryUnited States
CitySan Jose, CA


  • Optical properties
  • Temperature
  • Visible spectra

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics


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