TY - JOUR
T1 - Electrochemical detection of allergen in small-volume whole blood using an array microelectrode
T2 - A simple method for detection of allergic reaction
AU - Okochi, Mina
AU - Yokouchi, Hiroko
AU - Nakamura, Noriyuki
AU - Matsunaga, Tadashi
PY - 1999/11/20
Y1 - 1999/11/20
N2 - A safe, simplified, and rapid method for detection of allergen has been developed. Serotonin, a chemical mediator secreted during an allergic reaction, was used as a marker in electrochemical detection. A 20-μL drop of whole blood was used for the electrochemical detection of allergen using an array microelectrode. When cyclic voltammetry was carried out on whole blood samples containing 1 μg/mL serotonin, an anodic peak current appeared at around 350 mV versus a silver/silver chloride electrode using a Nation-coated array microelectrode. Allergen was selectively detected using whole blood samples by applying a constant potential of 350 mv after 40 min incubation with addition of allergen. The results obtained by the electrochemical detection method correlated well with the diagnosis obtained from the amount of IgE antibody.
AB - A safe, simplified, and rapid method for detection of allergen has been developed. Serotonin, a chemical mediator secreted during an allergic reaction, was used as a marker in electrochemical detection. A 20-μL drop of whole blood was used for the electrochemical detection of allergen using an array microelectrode. When cyclic voltammetry was carried out on whole blood samples containing 1 μg/mL serotonin, an anodic peak current appeared at around 350 mV versus a silver/silver chloride electrode using a Nation-coated array microelectrode. Allergen was selectively detected using whole blood samples by applying a constant potential of 350 mv after 40 min incubation with addition of allergen. The results obtained by the electrochemical detection method correlated well with the diagnosis obtained from the amount of IgE antibody.
KW - Array microelectrode
KW - Biosensor
KW - Electrochemical detection
KW - Serotonin
KW - Whole blood
UR - http://www.scopus.com/inward/record.url?scp=0033589833&partnerID=8YFLogxK
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U2 - 10.1002/(SICI)1097-0290(19991120)65:4<480::AID-BIT13>3.0.CO;2-F
DO - 10.1002/(SICI)1097-0290(19991120)65:4<480::AID-BIT13>3.0.CO;2-F
M3 - Article
C2 - 10506424
AN - SCOPUS:0033589833
SN - 0006-3592
VL - 65
SP - 480
EP - 484
JO - Biotechnology and Bioengineering
JF - Biotechnology and Bioengineering
IS - 4
ER -