Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G

Tae Kyu Lim; Noriyuki Nakamura; Masateru Ikehata; Tadashi Matsunaga

Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G

Tae Kyu Lim, Noriyuki Nakamura, Masateru Ikehata, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L = dm³). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.

Original language	English
Pages (from-to)	872-874
Number of pages	3
Journal	Electrochemistry
Volume	68
Issue number	11
Publication status	Published - 2000 Nov
Externally published	Yes

Keywords

Capillary Column
Electrochemical Flow Immunoassay
Ferrocene Conjugated IgG
HCG

ASJC Scopus subject areas

Electrochemistry

Cite this

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title = "Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G",

abstract = "This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L = dm3). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.",

keywords = "Capillary Column, Electrochemical Flow Immunoassay, Ferrocene Conjugated IgG, HCG",

author = "Lim, {Tae Kyu} and Noriyuki Nakamura and Masateru Ikehata and Tadashi Matsunaga",

year = "2000",

month = nov,

language = "English",

volume = "68",

pages = "872--874",

journal = "Electrochemistry",

issn = "1344-3542",

publisher = "Electrochemical Society of Japan",

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TY - JOUR

T1 - Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G

AU - Lim, Tae Kyu

AU - Nakamura, Noriyuki

AU - Ikehata, Masateru

AU - Matsunaga, Tadashi

PY - 2000/11

Y1 - 2000/11

N2 - This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L = dm3). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.

AB - This paper describes a miniaturized flow immunoassay system. Ferrocene (Fc) conjugated with anti-HCG immunoglobulin G (IgG) antibody (Fc-IgG) was prepared and used as a novel analytical reagent. The system consists of the immunoreaction part, the capillary column packed with cation exchange resin, and the flow cell for electrochemical detection of Fc-IgG. The assay yielded a linear relationship between signal and HCG concentration in the range of 0-2000 mIU/mL (L = dm3). This electrochemical flow immunoassay requires minute quantities of serum and generates highly reproducible results.

KW - Capillary Column

KW - Electrochemical Flow Immunoassay

KW - Ferrocene Conjugated IgG

KW - HCG

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M3 - Article

AN - SCOPUS:0012105058

SN - 1344-3542

VL - 68

SP - 872

EP - 874

JO - Electrochemistry

JF - Electrochemistry

IS - 11

ER -

Electrochemical Flow Immunoassay Using Capillary Column and Ferrocene Conjugated Immunoglobulin G

Abstract

Keywords

ASJC Scopus subject areas

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