Electrospun nanofiber biosensor for measuring glucose concentration

An amperometric biosensor was fabricated using electospun composite nanofiber membra ne which are consisted of among polyaniline- polystylene nanofibers. Polyaniline was reacted with camphorsulfonic acid to produce a salt, which was then dissolved in chloroform containing polystyrene. By electrospinning this polymer solution, nanofibers were formed and collected at the counter electrodes. Glucose oxidase was immobilized onto these nanofiber matt using an electrostatic layer-by-layer adsorption technique. In this method, pory(diallyldimethylammonium chloride) was used as the counter ion source. The electrical property of this composite nariofib er was investigated from these nanofibers as a working electrode, and used to measure the glucose concentration accurately.