Encapsulation of Hb into unsaturated lipid vesicles and γ‐ray polymerization

Hiromi Sakai; Shinji Takeoka; Hiroaki Yokohama; Hiroyuki Nishide; Eishun Tsuchida

doi:10.1002/pat.1992.220030704

Encapsulation of Hb into unsaturated lipid vesicles and γ‐ray polymerization

Hiromi Sakai, Shinji Takeoka, Hiroaki Yokohama, Hiroyuki Nishide, Eishun Tsuchida^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

A carbonyl hemoglobin (HbCO) solution was stirred with a mixed powder of polymerizable 1,2‐bis(2,4‐octadecadienoyl)‐sn‐glycero‐3‐phosphocholine (DODPC), cholesterol and stearic acid (7/7/2 by mol). The mixture was extruded through polycarbonate membrane filters (final pore size = 0.2 μm Ø). The average diameter of the resulting vesicles was 203 ± 39 nm. The [Hb]/[Lipid] ratio (the weight ratio of Hb in vesicle to lipid) increased with the Hb concentration, and decreased with the NaCl concentration. A maximum [Hb]/[lipid] ratio was observed at pH 6.9, which was the same as the isoelectric point of Hb. The vesicles were stabilized by γ‐irradiation (⁶⁰Co) because the bilayer lipids bound each other to yield polyphospholipids. Denaturation of Hb by γ‐irradiation was not detected. These polyphospholipid vesicles encapsulating Hb were stable even against the freeze–thaw cycles and the freeze‐drying procedure.

Original language	English
Pages (from-to)	389-394
Number of pages	6
Journal	Polymers for Advanced Technologies
Volume	3
Issue number	7
DOIs	https://doi.org/10.1002/pat.1992.220030704
Publication status	Published - 1992 Nov

Keywords

Blood substitute
Encapsulation
Hemoglobin
Lipid vesicle
γ‐Ray polymerization

ASJC Scopus subject areas

Polymers and Plastics

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10.1002/pat.1992.220030704

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title = "Encapsulation of Hb into unsaturated lipid vesicles and γ‐ray polymerization",

abstract = "A carbonyl hemoglobin (HbCO) solution was stirred with a mixed powder of polymerizable 1,2‐bis(2,4‐octadecadienoyl)‐sn‐glycero‐3‐phosphocholine (DODPC), cholesterol and stearic acid (7/7/2 by mol). The mixture was extruded through polycarbonate membrane filters (final pore size = 0.2 μm {\O}). The average diameter of the resulting vesicles was 203 ± 39 nm. The [Hb]/[Lipid] ratio (the weight ratio of Hb in vesicle to lipid) increased with the Hb concentration, and decreased with the NaCl concentration. A maximum [Hb]/[lipid] ratio was observed at pH 6.9, which was the same as the isoelectric point of Hb. The vesicles were stabilized by γ‐irradiation (60Co) because the bilayer lipids bound each other to yield polyphospholipids. Denaturation of Hb by γ‐irradiation was not detected. These polyphospholipid vesicles encapsulating Hb were stable even against the freeze–thaw cycles and the freeze‐drying procedure.",

keywords = "Blood substitute, Encapsulation, Hemoglobin, Lipid vesicle, γ‐Ray polymerization",

author = "Hiromi Sakai and Shinji Takeoka and Hiroaki Yokohama and Hiroyuki Nishide and Eishun Tsuchida",

year = "1992",

month = nov,

doi = "10.1002/pat.1992.220030704",

language = "English",

volume = "3",

pages = "389--394",

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TY - JOUR

T1 - Encapsulation of Hb into unsaturated lipid vesicles and γ‐ray polymerization

AU - Sakai, Hiromi

AU - Takeoka, Shinji

AU - Yokohama, Hiroaki

AU - Nishide, Hiroyuki

AU - Tsuchida, Eishun

PY - 1992/11

Y1 - 1992/11

N2 - A carbonyl hemoglobin (HbCO) solution was stirred with a mixed powder of polymerizable 1,2‐bis(2,4‐octadecadienoyl)‐sn‐glycero‐3‐phosphocholine (DODPC), cholesterol and stearic acid (7/7/2 by mol). The mixture was extruded through polycarbonate membrane filters (final pore size = 0.2 μm Ø). The average diameter of the resulting vesicles was 203 ± 39 nm. The [Hb]/[Lipid] ratio (the weight ratio of Hb in vesicle to lipid) increased with the Hb concentration, and decreased with the NaCl concentration. A maximum [Hb]/[lipid] ratio was observed at pH 6.9, which was the same as the isoelectric point of Hb. The vesicles were stabilized by γ‐irradiation (60Co) because the bilayer lipids bound each other to yield polyphospholipids. Denaturation of Hb by γ‐irradiation was not detected. These polyphospholipid vesicles encapsulating Hb were stable even against the freeze–thaw cycles and the freeze‐drying procedure.

AB - A carbonyl hemoglobin (HbCO) solution was stirred with a mixed powder of polymerizable 1,2‐bis(2,4‐octadecadienoyl)‐sn‐glycero‐3‐phosphocholine (DODPC), cholesterol and stearic acid (7/7/2 by mol). The mixture was extruded through polycarbonate membrane filters (final pore size = 0.2 μm Ø). The average diameter of the resulting vesicles was 203 ± 39 nm. The [Hb]/[Lipid] ratio (the weight ratio of Hb in vesicle to lipid) increased with the Hb concentration, and decreased with the NaCl concentration. A maximum [Hb]/[lipid] ratio was observed at pH 6.9, which was the same as the isoelectric point of Hb. The vesicles were stabilized by γ‐irradiation (60Co) because the bilayer lipids bound each other to yield polyphospholipids. Denaturation of Hb by γ‐irradiation was not detected. These polyphospholipid vesicles encapsulating Hb were stable even against the freeze–thaw cycles and the freeze‐drying procedure.

KW - Blood substitute

KW - Encapsulation

KW - Hemoglobin

KW - Lipid vesicle

KW - γ‐Ray polymerization

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DO - 10.1002/pat.1992.220030704

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JO - Polymers for Advanced Technologies

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ER -

Encapsulation of Hb into unsaturated lipid vesicles and γ‐ray polymerization

Abstract

Keywords

ASJC Scopus subject areas

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