Engineering proteins that bind, move, make and break DNA

Cynthia H. Collins*, Yohei Yokobayashi, Daisuke Umeno, Frances H. Arnold

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)


Recent protein engineering efforts have generated artificial transcription factors that bind new target DNA sequences and enzymes that modify DNA at new target sites. Zinc-finger-based transcription factors are favored targets for design; important technological advances in their construction and numerous biotechnological applications have been reported. Other notable advances include the generation of endonucleases and recombinases with altered specificities, made by innovative combinatorial and evolutionary protein engineering strategies. An unexpectedly high tolerance to mutation in the active sites of DNA polymerases is being exploited to engineer polymerases to incorporate artificial nucleotides or to display other, nonnatural activities.

Original languageEnglish
Pages (from-to)371-378
Number of pages8
JournalCurrent Opinion in Biotechnology
Issue number4
Publication statusPublished - 2003 Aug
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering


Dive into the research topics of 'Engineering proteins that bind, move, make and break DNA'. Together they form a unique fingerprint.

Cite this