Enhancement and repression of cyanide-insensitive respiration in Aspergillus niger

Kohtaro Kirimura; Tomoko Matsui; Shuji Sugano; Shoji Usami

doi:10.1016/0378-1097(96)00230-3

Enhancement and repression of cyanide-insensitive respiration in Aspergillus niger

Kohtaro Kirimura^*, Tomoko Matsui, Shuji Sugano, Shoji Usami

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

The biosynthesis of the alternative oxidase protein consisting of cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in Aspergillus niger was investigated. Cyanide-insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide, emetine, puromycin (cytosolic translation inhibitors), carbonylcyanide-m-chlorophenylhydrazone (uncoupler) and actinomycin D (transcription inhibitor). These results show that the alternative oxidase protein of A. niger was synthesized de novo in cytosol and transported in mitochondria.

Original language	English
Pages (from-to)	251-254
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	141
Issue number	2-3
DOIs	https://doi.org/10.1016/0378-1097(96)00230-3
Publication status	Published - 1996 Aug 1

Keywords

Alternative oxidase
Antimycin A
Aspergillus niger
Cyanide-insensitive respiration
Salicylhydroxamic acid

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/0378-1097(96)00230-3

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abstract = "The biosynthesis of the alternative oxidase protein consisting of cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in Aspergillus niger was investigated. Cyanide-insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide, emetine, puromycin (cytosolic translation inhibitors), carbonylcyanide-m-chlorophenylhydrazone (uncoupler) and actinomycin D (transcription inhibitor). These results show that the alternative oxidase protein of A. niger was synthesized de novo in cytosol and transported in mitochondria.",

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AU - Kirimura, Kohtaro

AU - Matsui, Tomoko

AU - Sugano, Shuji

AU - Usami, Shoji

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N2 - The biosynthesis of the alternative oxidase protein consisting of cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in Aspergillus niger was investigated. Cyanide-insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide, emetine, puromycin (cytosolic translation inhibitors), carbonylcyanide-m-chlorophenylhydrazone (uncoupler) and actinomycin D (transcription inhibitor). These results show that the alternative oxidase protein of A. niger was synthesized de novo in cytosol and transported in mitochondria.

AB - The biosynthesis of the alternative oxidase protein consisting of cyanide-insensitive and salicylhydroxamic acid-sensitive respiration in Aspergillus niger was investigated. Cyanide-insensitive respiration was enhanced by addition of antimycin A to the incubation mixture, but repressed by the addition of cycloheximide, emetine, puromycin (cytosolic translation inhibitors), carbonylcyanide-m-chlorophenylhydrazone (uncoupler) and actinomycin D (transcription inhibitor). These results show that the alternative oxidase protein of A. niger was synthesized de novo in cytosol and transported in mitochondria.

KW - Alternative oxidase

KW - Antimycin A

KW - Aspergillus niger

KW - Cyanide-insensitive respiration

KW - Salicylhydroxamic acid

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Enhancement and repression of cyanide-insensitive respiration in Aspergillus niger

Abstract

Keywords

ASJC Scopus subject areas

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