Enhancement of poly(arginyl-histidine) production by Verticillium kibiense E18

Ikumi Kurihara, Yoshitaka Ishii, Kohtaro Kirimura, Kuniki Kino*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


An ergot fungus Verticillium kibiense E18 produced two cationic peptides, ε-poly-l-lysine (ePL) and poly(l-arginyl-d-histidine) (PRH). The ePL was used as a food preservative, and it was expected that PRH would be used as a novel material, such as cationic and antimicrobial peptide. To enhance PRH production of strain E18, various culture conditions were investigated. Glucose was a suitable carbon source for PRH production, although glycerol was a suitable carbon source for growth. The cultivation temperature significantly influenced both cell growth and PRH production. The optimal temperatures for cell growth and PRH production were 28 and 30 °C, respectively. Moreover, strain E18 produced more PRH when an additional 5.0 μg/L FeSO4·7H2O was added to the production medium. Under optimal conditions, strain E18 enhanced PRH production, while suppressing ePL production. The maximum PRH production was 183.9 mg/L, which is approximately 60-fold higher than that of the initial culture condition.

Original languageEnglish
Pages (from-to)270-275
Number of pages6
JournalBiochemical Engineering Journal
Issue number3
Publication statusPublished - 2008 Dec 1


  • Antimicrobial peptide
  • Cationic peptide
  • Culture conditions
  • Poly(amino acid)
  • Poly(arginyl-histidine)
  • Verticillium kibiense

ASJC Scopus subject areas

  • Biotechnology
  • Environmental Engineering
  • Bioengineering
  • Biomedical Engineering


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