TY - JOUR
T1 - Enhancement of poly(arginyl-histidine) production by Verticillium kibiense E18
AU - Kurihara, Ikumi
AU - Ishii, Yoshitaka
AU - Kirimura, Kohtaro
AU - Kino, Kuniki
N1 - Funding Information:
We are deeply grateful to Dr. Masanobu Nishikawa (Research Institute for Biological Science, Okayama) for distribution of V. kibiense E18, and to Chisso Corporation for providing synthetic PRH and supports. This work was partly supported by Encouraging Development Strategic Research Centers Program, the Special Coordination Funds for Promoting Science and Technology (Establishment of Consolidated Research Institute for Advanced Science and Medical Care) from MEXT, Japan.
PY - 2008/12/1
Y1 - 2008/12/1
N2 - An ergot fungus Verticillium kibiense E18 produced two cationic peptides, ε-poly-l-lysine (ePL) and poly(l-arginyl-d-histidine) (PRH). The ePL was used as a food preservative, and it was expected that PRH would be used as a novel material, such as cationic and antimicrobial peptide. To enhance PRH production of strain E18, various culture conditions were investigated. Glucose was a suitable carbon source for PRH production, although glycerol was a suitable carbon source for growth. The cultivation temperature significantly influenced both cell growth and PRH production. The optimal temperatures for cell growth and PRH production were 28 and 30 °C, respectively. Moreover, strain E18 produced more PRH when an additional 5.0 μg/L FeSO4·7H2O was added to the production medium. Under optimal conditions, strain E18 enhanced PRH production, while suppressing ePL production. The maximum PRH production was 183.9 mg/L, which is approximately 60-fold higher than that of the initial culture condition.
AB - An ergot fungus Verticillium kibiense E18 produced two cationic peptides, ε-poly-l-lysine (ePL) and poly(l-arginyl-d-histidine) (PRH). The ePL was used as a food preservative, and it was expected that PRH would be used as a novel material, such as cationic and antimicrobial peptide. To enhance PRH production of strain E18, various culture conditions were investigated. Glucose was a suitable carbon source for PRH production, although glycerol was a suitable carbon source for growth. The cultivation temperature significantly influenced both cell growth and PRH production. The optimal temperatures for cell growth and PRH production were 28 and 30 °C, respectively. Moreover, strain E18 produced more PRH when an additional 5.0 μg/L FeSO4·7H2O was added to the production medium. Under optimal conditions, strain E18 enhanced PRH production, while suppressing ePL production. The maximum PRH production was 183.9 mg/L, which is approximately 60-fold higher than that of the initial culture condition.
KW - Antimicrobial peptide
KW - Cationic peptide
KW - Culture conditions
KW - Poly(amino acid)
KW - Poly(arginyl-histidine)
KW - Verticillium kibiense
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U2 - 10.1016/j.bej.2008.07.007
DO - 10.1016/j.bej.2008.07.007
M3 - Article
AN - SCOPUS:52049098891
SN - 1369-703X
VL - 42
SP - 270
EP - 275
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
IS - 3
ER -