Enhancer of human polyoma JC virus contains nuclear factor I-binding sequences; analysis using mouse brain nuclear extracts

Taka aki Tamura; Takafumi Inoue; Kyosuke Nagata; Katsuhiko Mikoshiba

doi:10.1016/S0006-291X(88)80265-1

Enhancer of human polyoma JC virus contains nuclear factor I-binding sequences; analysis using mouse brain nuclear extracts

Taka aki Tamura^*, Takafumi Inoue, Kyosuke Nagata, Katsuhiko Mikoshiba

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

Neurotrophic human JC virus carries a 98 bp duplicate enhancer responsible for tissue-specific gene expression. DNase I footprinting studies using mouse brain nuclear extracts revealed weak (pseudo NFI motif) and strong (NFI motif) nuclear factor I-binding sequences just upstream (at 229) and in the middle (at 156 and 58) of the enhancer, respectively. In vitro transcription driven in brain extracts demonstrated that the NFI motif is a possible transcription control element. Together with previous observations(Khalili,K., Rappaport,J. and Khoury,G.(1988) EMBO J. 7, 1205-1210 (20)), the NFI motif is suggested to play an important role in early gene expression of JCV.

Original language	English
Pages (from-to)	419-425
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	157
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(88)80265-1
Publication status	Published - 1988 Dec 15
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{09bd98f5cb36446196aae3110062cb2d,

title = "Enhancer of human polyoma JC virus contains nuclear factor I-binding sequences; analysis using mouse brain nuclear extracts",

abstract = "Neurotrophic human JC virus carries a 98 bp duplicate enhancer responsible for tissue-specific gene expression. DNase I footprinting studies using mouse brain nuclear extracts revealed weak (pseudo NFI motif) and strong (NFI motif) nuclear factor I-binding sequences just upstream (at 229) and in the middle (at 156 and 58) of the enhancer, respectively. In vitro transcription driven in brain extracts demonstrated that the NFI motif is a possible transcription control element. Together with previous observations(Khalili,K., Rappaport,J. and Khoury,G.(1988) EMBO J. 7, 1205-1210 (20)), the NFI motif is suggested to play an important role in early gene expression of JCV.",

author = "Tamura, {Taka aki} and Takafumi Inoue and Kyosuke Nagata and Katsuhiko Mikoshiba",

note = "Funding Information: We wish to thank Drs. K.Ikenaka, H.Okano, T.Furuichi, M.Miura and A.Ao.yama for helpful discussion, and Y.Ohya for technical assistance. We also thank Dr. R.Frisque for providing cloned JCV DNA. This work was supported by a grant from the Japanese ministry of Education, Science and Culture.",

year = "1988",

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doi = "10.1016/S0006-291X(88)80265-1",

language = "English",

volume = "157",

pages = "419--425",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Enhancer of human polyoma JC virus contains nuclear factor I-binding sequences; analysis using mouse brain nuclear extracts

AU - Tamura, Taka aki

AU - Inoue, Takafumi

AU - Nagata, Kyosuke

AU - Mikoshiba, Katsuhiko

N1 - Funding Information: We wish to thank Drs. K.Ikenaka, H.Okano, T.Furuichi, M.Miura and A.Ao.yama for helpful discussion, and Y.Ohya for technical assistance. We also thank Dr. R.Frisque for providing cloned JCV DNA. This work was supported by a grant from the Japanese ministry of Education, Science and Culture.

PY - 1988/12/15

Y1 - 1988/12/15

N2 - Neurotrophic human JC virus carries a 98 bp duplicate enhancer responsible for tissue-specific gene expression. DNase I footprinting studies using mouse brain nuclear extracts revealed weak (pseudo NFI motif) and strong (NFI motif) nuclear factor I-binding sequences just upstream (at 229) and in the middle (at 156 and 58) of the enhancer, respectively. In vitro transcription driven in brain extracts demonstrated that the NFI motif is a possible transcription control element. Together with previous observations(Khalili,K., Rappaport,J. and Khoury,G.(1988) EMBO J. 7, 1205-1210 (20)), the NFI motif is suggested to play an important role in early gene expression of JCV.

AB - Neurotrophic human JC virus carries a 98 bp duplicate enhancer responsible for tissue-specific gene expression. DNase I footprinting studies using mouse brain nuclear extracts revealed weak (pseudo NFI motif) and strong (NFI motif) nuclear factor I-binding sequences just upstream (at 229) and in the middle (at 156 and 58) of the enhancer, respectively. In vitro transcription driven in brain extracts demonstrated that the NFI motif is a possible transcription control element. Together with previous observations(Khalili,K., Rappaport,J. and Khoury,G.(1988) EMBO J. 7, 1205-1210 (20)), the NFI motif is suggested to play an important role in early gene expression of JCV.

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Enhancer of human polyoma JC virus contains nuclear factor I-binding sequences; analysis using mouse brain nuclear extracts

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