TY - JOUR
T1 - Enzymatic Bioluminescence Modulation with an ATP Synthase Integrated Biotransducer
AU - Chen, Yukun
AU - Cui, Mingyin
AU - Lin, Chenliang
AU - Liu, Bingfu
AU - Mitome, Noriyo
AU - Miyake, Takeo
N1 - Funding Information:
T.M. conceived the study. T.M. and N.M. designed the experiments. Y.C., N.M., and M.C. performed the experiments. T.M., Y.C., and N.M. analyzed the data. Y.C. and N.M. prepared the ATP synthase. T.M., Y.C., and N.M. wrote the manuscript. This work was supported by the JST, PRESTO Grant Number: JPMJPR20B8, and a Grant‐in‐Aid for Scientific Research (B). A portion of this work was conducted at the Kitakyushu Foundation for the Advancement of Industry, Science and Technology, Semiconductor Center, and supported by the “Nanotechnology Platform Program” of the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) of Japan. N.M. was supported by the Cooperative Research Program of “NJRC Mater. & Dev.” The authors thank Dr. Toshiharu Suzuki and Dr. Masasuke Yoshida for providing us with the expression plasmid for FoF1 ATP synthase.
Publisher Copyright:
© 2021 Wiley-VCH GmbH.
PY - 2022/1
Y1 - 2022/1
N2 - Ion carriers play an important role in biology in terms of control of a biological system, whereas electron carriers are key to engineered systems. Therefore, a biodevice requires an electron–ion coupling mechanism that can transmit an ion signal from a device to a biological system via an external voltage. In this study, a protonic biodevice is demonstrated that translates the proton signals from the device into an enzyme cascade by means of an ATP synthase and bioluminescent luciferase. The proton signals can be modulated through the applied potential to a sulfonated polyaniline biotransducer, with such protonic modulation controlling ATP synthesis at the ATP synthase membrane, followed by bioluminescence in the luciferin–luciferase reaction. The present electron–proton-transportation platform provides a new means of controlling biological cascade reactions, with proton signals mediated by an external voltage.
AB - Ion carriers play an important role in biology in terms of control of a biological system, whereas electron carriers are key to engineered systems. Therefore, a biodevice requires an electron–ion coupling mechanism that can transmit an ion signal from a device to a biological system via an external voltage. In this study, a protonic biodevice is demonstrated that translates the proton signals from the device into an enzyme cascade by means of an ATP synthase and bioluminescent luciferase. The proton signals can be modulated through the applied potential to a sulfonated polyaniline biotransducer, with such protonic modulation controlling ATP synthesis at the ATP synthase membrane, followed by bioluminescence in the luciferin–luciferase reaction. The present electron–proton-transportation platform provides a new means of controlling biological cascade reactions, with proton signals mediated by an external voltage.
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U2 - 10.1002/admt.202100729
DO - 10.1002/admt.202100729
M3 - Article
AN - SCOPUS:85114686369
SN - 2365-709X
VL - 7
JO - Advanced Materials Technologies
JF - Advanced Materials Technologies
IS - 1
M1 - 2100729
ER -