Enzymatic reduction of synthetic polymer-bound hemin derivatives: efficient method to prepare a heme-oxygen adduct in cooled aqueous medium

Nobuo Uotani, Etsuo Hasegawa, Hiroyuki Nishide, Eishun Tsuchida*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Synthetic polymer-bound hemin (iron(III) protoporphyrin IX) derivatives were effectively reduced by ferredoxin and ferredoxin-NADP reductase system. The resultant polymer-bound heme (iron(II) protoporphyrin IX) derivatives formed oxygen adducts with a lifetime of ca. 1 hr in aqueous solution at -30°C. The reduction rate is discussed in terms of the structure of the hemin derivatives.

Original languageEnglish
Pages (from-to)85-89
Number of pages5
JournalJournal of Inorganic Biochemistry
Volume22
Issue number2
DOIs
Publication statusPublished - 1984

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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