Enzymatic synthesis of oligonucleotides of defined sequence: The "single addition" of 2'(3 )-o-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase

Yo Kikuchi; Keiko Hirai; Kinumi Someno; Kenji Sakaguchi

Enzymatic synthesis of oligonucleotides of defined sequence: The "single addition" of 2'(3 )-o-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase

Yo Kikuchi^*, Keiko Hirai, Kinumi Someno, Kenji Sakaguchi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Several oligonucleotides of defined sequence were synthesized using 2'(3')-O-dihydrocinnamoyl-nucleoside 5'-diphosphates (DHC-NDP) as substrates for polynucleotide phosphorylase [EC 2. 7. 7. 8] from Thermus thermophilus. The enzyme catalyzed the transfer of one nucleotidyl residue from each of the 2'(3')-O-dihydrocinnamoyl esters of CDP, UDP, and GDP to the 3'-terminus of the primer triadenosine diphosphate, (Ap)₂A. The products were shown to be (Ap)₂C, (Ap)₃U, and (Ap)₈G by enzymatic analysis.

Original language	English
Pages (from-to)	33-37
Number of pages	5
Journal	Journal of Biochemistry
Volume	80
Issue number	1
Publication status	Published - 1976 Jul
Externally published	Yes

ASJC Scopus subject areas

Statistics, Probability and Uncertainty
Applied Mathematics
Physiology (medical)
Radiology Nuclear Medicine and imaging
Molecular Biology
Biochemistry

Fingerprint

Dive into the research topics of 'Enzymatic synthesis of oligonucleotides of defined sequence: The "single addition" of 2'(3 )-o-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase'. Together they form a unique fingerprint.

Cite this

Enzymatic synthesis of oligonucleotides of defined sequence: The "single addition" of 2'(3 )-o-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase. / Kikuchi, Yo; Hirai, Keiko; Someno, Kinumi et al.
In: Journal of Biochemistry, Vol. 80, No. 1, 07.1976, p. 33-37.

Research output: Contribution to journal › Article › peer-review

@article{d174e8c72da846b680760449e12279bb,

title = "Enzymatic synthesis of oligonucleotides of defined sequence: The {"}single addition{"} of 2'(3 )-o-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase",

abstract = "Several oligonucleotides of defined sequence were synthesized using 2'(3')-O-dihydrocinnamoyl-nucleoside 5'-diphosphates (DHC-NDP) as substrates for polynucleotide phosphorylase [EC 2. 7. 7. 8] from Thermus thermophilus. The enzyme catalyzed the transfer of one nucleotidyl residue from each of the 2'(3')-O-dihydrocinnamoyl esters of CDP, UDP, and GDP to the 3'-terminus of the primer triadenosine diphosphate, (Ap)2A. The products were shown to be (Ap)2C, (Ap)3U, and (Ap)8G by enzymatic analysis.",

author = "Yo Kikuchi and Keiko Hirai and Kinumi Someno and Kenji Sakaguchi",

year = "1976",

month = jul,

language = "English",

volume = "80",

pages = "33--37",

journal = "Journal of Biochemistry",

issn = "0021-924X",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - Enzymatic synthesis of oligonucleotides of defined sequence

T2 - The "single addition" of 2'(3 )-o-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase

AU - Kikuchi, Yo

AU - Hirai, Keiko

AU - Someno, Kinumi

AU - Sakaguchi, Kenji

PY - 1976/7

Y1 - 1976/7

N2 - Several oligonucleotides of defined sequence were synthesized using 2'(3')-O-dihydrocinnamoyl-nucleoside 5'-diphosphates (DHC-NDP) as substrates for polynucleotide phosphorylase [EC 2. 7. 7. 8] from Thermus thermophilus. The enzyme catalyzed the transfer of one nucleotidyl residue from each of the 2'(3')-O-dihydrocinnamoyl esters of CDP, UDP, and GDP to the 3'-terminus of the primer triadenosine diphosphate, (Ap)2A. The products were shown to be (Ap)2C, (Ap)3U, and (Ap)8G by enzymatic analysis.

AB - Several oligonucleotides of defined sequence were synthesized using 2'(3')-O-dihydrocinnamoyl-nucleoside 5'-diphosphates (DHC-NDP) as substrates for polynucleotide phosphorylase [EC 2. 7. 7. 8] from Thermus thermophilus. The enzyme catalyzed the transfer of one nucleotidyl residue from each of the 2'(3')-O-dihydrocinnamoyl esters of CDP, UDP, and GDP to the 3'-terminus of the primer triadenosine diphosphate, (Ap)2A. The products were shown to be (Ap)2C, (Ap)3U, and (Ap)8G by enzymatic analysis.

UR - http://www.scopus.com/inward/record.url?scp=0017169246&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017169246&partnerID=8YFLogxK

M3 - Article

C2 - 9379

AN - SCOPUS:0017169246

SN - 0021-924X

VL - 80

SP - 33

EP - 37

JO - Journal of Biochemistry

JF - Journal of Biochemistry

IS - 1

ER -

Enzymatic synthesis of oligonucleotides of defined sequence: The "single addition" of 2'(3 )-o-dihydrocinnamoyl-nucleoside 5'-diphosphate to a primer oligonucleotide catalyzed by a thermophilic polynucleotide phosphorylase

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this