Abstract
We have demonstrated the efficacy of a microfluidic medium exchange method for single cells using passive centrifugal force of a rotating microfluidic-chip based platform. At the boundary of two laminar flows at the gathering area of two microfluidic pathways in a Y-shape, the cells were successfully transported from one laminar flow to the other, without mixing the two microfluidic mediums of the two laminar flows during cell transportation, within 5 s with 1 g (150 rpm) to 36.3 g (900 rpm) acceleration, with 93.5% efficiency. The results indicate that this is one of the most simple and precise tools for exchanging medium in the shortest amount of time.
| Original language | English |
|---|---|
| Pages (from-to) | 819-827 |
| Number of pages | 9 |
| Journal | International journal of molecular sciences |
| Volume | 13 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2012 Jan |
| Externally published | Yes |
Keywords
- Centrifugal force
- Environmental control
- Medium exchange
- Microfluidic chip
- On-chip cellomics
- Single cell
- Y-shape platform
ASJC Scopus subject areas
- Catalysis
- Molecular Biology
- Spectroscopy
- Computer Science Applications
- Physical and Theoretical Chemistry
- Organic Chemistry
- Inorganic Chemistry