Evaluation of cancer cell deformability by microcavity array

Tomoko Yoshino; Tsuyoshi Tanaka; Seita Nakamura; Ryo Negishi; Nozomi Shionoiri; Masahito Hosokawa; Tadashi Matsunaga

doi:10.1016/j.ab.2016.12.026

Evaluation of cancer cell deformability by microcavity array

Tomoko Yoshino^*, Tsuyoshi Tanaka, Seita Nakamura, Ryo Negishi, Nozomi Shionoiri, Masahito Hosokawa, Tadashi Matsunaga

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A cell entrapment device consisting of a microcavity array was used to analyze the deformability of MCF-10 human breast epithelial and MCF-7 human breast cancer cell lines by confocal laser scanning microscopy. Entrapment of up to 8 × 10³ cells was achieved within 3 min. Protrusions were formed at the bottom surface of the array with a pore size of 3 μm. Protrusion length increased at higher filtration pressures and could be used to distinguish between MCF-7 and MCF-10 cells. These results indicate that our system is useful for high-throughput deformability analysis of cancer cells, which can provide insight into the mechanisms underlying tumor cell malignancy.

Original language	English
Pages (from-to)	16-21
Number of pages	6
Journal	Analytical Biochemistry
Volume	520
DOIs	https://doi.org/10.1016/j.ab.2016.12.026
Publication status	Published - 2017 Mar 1
Externally published	Yes

Keywords

Breast cancer
Cell deformability

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.ab.2016.12.026

Cite this

@article{900b6b7463fb430e929ee5b6ea188e9a,

title = "Evaluation of cancer cell deformability by microcavity array",

abstract = "A cell entrapment device consisting of a microcavity array was used to analyze the deformability of MCF-10 human breast epithelial and MCF-7 human breast cancer cell lines by confocal laser scanning microscopy. Entrapment of up to 8 × 103 cells was achieved within 3 min. Protrusions were formed at the bottom surface of the array with a pore size of 3 μm. Protrusion length increased at higher filtration pressures and could be used to distinguish between MCF-7 and MCF-10 cells. These results indicate that our system is useful for high-throughput deformability analysis of cancer cells, which can provide insight into the mechanisms underlying tumor cell malignancy.",

keywords = "Breast cancer, Cell deformability",

author = "Tomoko Yoshino and Tsuyoshi Tanaka and Seita Nakamura and Ryo Negishi and Nozomi Shionoiri and Masahito Hosokawa and Tadashi Matsunaga",

year = "2017",

month = mar,

day = "1",

doi = "10.1016/j.ab.2016.12.026",

language = "English",

volume = "520",

pages = "16--21",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Evaluation of cancer cell deformability by microcavity array

AU - Yoshino, Tomoko

AU - Tanaka, Tsuyoshi

AU - Nakamura, Seita

AU - Negishi, Ryo

AU - Shionoiri, Nozomi

AU - Hosokawa, Masahito

AU - Matsunaga, Tadashi

PY - 2017/3/1

Y1 - 2017/3/1

N2 - A cell entrapment device consisting of a microcavity array was used to analyze the deformability of MCF-10 human breast epithelial and MCF-7 human breast cancer cell lines by confocal laser scanning microscopy. Entrapment of up to 8 × 103 cells was achieved within 3 min. Protrusions were formed at the bottom surface of the array with a pore size of 3 μm. Protrusion length increased at higher filtration pressures and could be used to distinguish between MCF-7 and MCF-10 cells. These results indicate that our system is useful for high-throughput deformability analysis of cancer cells, which can provide insight into the mechanisms underlying tumor cell malignancy.

AB - A cell entrapment device consisting of a microcavity array was used to analyze the deformability of MCF-10 human breast epithelial and MCF-7 human breast cancer cell lines by confocal laser scanning microscopy. Entrapment of up to 8 × 103 cells was achieved within 3 min. Protrusions were formed at the bottom surface of the array with a pore size of 3 μm. Protrusion length increased at higher filtration pressures and could be used to distinguish between MCF-7 and MCF-10 cells. These results indicate that our system is useful for high-throughput deformability analysis of cancer cells, which can provide insight into the mechanisms underlying tumor cell malignancy.

KW - Breast cancer

KW - Cell deformability

UR - http://www.scopus.com/inward/record.url?scp=85008881038&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85008881038&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2016.12.026

DO - 10.1016/j.ab.2016.12.026

M3 - Article

C2 - 28057503

AN - SCOPUS:85008881038

SN - 0003-2697

VL - 520

SP - 16

EP - 21

JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -

Evaluation of cancer cell deformability by microcavity array

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this