@article{9ba1c50afcda4a8480aedf0ca43b0c5e,
title = "Excitatory Neuronal Hubs Configure Multisensory Integration of Slow Waves in Association Cortex",
abstract = "Multisensory integration (MSI) is a fundamental emergent property of the mammalian brain. During MSI, perceptual information encoded in patterned activity is processed in multimodal association cortex. The systems-level neuronal dynamics that coordinate MSI, however, are unknown. Here, we demonstrate intrinsic hub-like network activity in the association cortex that regulates MSI. We engineered calcium reporter mouse lines based on the fluorescence resonance energy transfer sensor yellow cameleon (YC2.60) expressed in excitatory or inhibitory neurons. In medial and parietal association cortex, we observed spontaneous slow waves that self-organized into hubs defined by long-range excitatory and local inhibitory circuits. Unlike directional source/sink-like flows in sensory areas, medial/parietal excitatory and inhibitory hubs had net-zero balanced inputs. Remarkably, multisensory stimulation triggered rapid phase-locking mainly of excitatory hub activity persisting for seconds after the stimulus offset. Therefore, association cortex tends to form balanced excitatory networks that configure slow-wave phase-locking for MSI. Video Abstract [Figure presented] Kuroki et al. performed cell-type-specific, wide-field FRET-based calcium imaging to visualize cortical network activity induced by multisensory inputs. They observed phase-locking of cortical slow waves in excitatory neuronal hubs in association cortical areas that may underlie multisensory integration.",
keywords = "association cortex, cortical slow waves, excitatory neuron, fluorescence resonance energy transfer, inhibitory neuron, mouse, multisensory integration, phase locking, spontaneous activity, wide-field calcium imaging",
author = "Satoshi Kuroki and Takamasa Yoshida and Hidekazu Tsutsui and Mizuho Iwama and Reiko Ando and Takayuki Michikawa and Atsushi Miyawaki and Toshio Ohshima and Shigeyoshi Itohara",
note = "Funding Information: We thank Charles Yokoyama for his great help in editing the manuscript. We thank Keiichi Kitajo for technical advice for data analysis. We thank Kei Sunouchi for the fabrication of the head plate and clamp. We thank Hiroki Hamanaka, Yuri Ishizu, and Joshua Johansen for providing the AAV-CaMKIIα-Cre template plasmid. We thank Roman Boehringer, Kentaro Tao, Satoshi Terada, Akihiro Shimbo, and Akira Masuda for technical advice for the in vivo electrophysiologic experiments. We thank Yuki Kobayashi for instruction on the viral purification, Kazuhiko Yamaguchi for critical comments, and all members of S.I.{\textquoteright}s laboratory for encouragement and help. The work was supported in part by Grant-in-Aid for Japan Society for the Promotion of Sciences (JSPS) Fellows ( 12J00301 ) to S.K., Grant-in-Aid for Young Scientists (B) from JSPS ( 25871135 ) to T.Y., the program for Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) from the Japan Agency for Medical Research and Development (AMED) to A.M. and S.I., the Ministry of Education, Culture, Sports, Science, and Technology (MEXT) -Supported Program for the Strategic Research Foundation at Private Universities, 2012–2016 to T.O., and Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program) initiated by the Council for Science and Technology Policy (CSTP) to S.I. Funding Information: We thank Charles Yokoyama for his great help in editing the manuscript. We thank Keiichi Kitajo for technical advice for data analysis. We thank Kei Sunouchi for the fabrication of the head plate and clamp. We thank Hiroki Hamanaka, Yuri Ishizu, and Joshua Johansen for providing the AAV-CaMKIIα-Cre template plasmid. We thank Roman Boehringer, Kentaro Tao, Satoshi Terada, Akihiro Shimbo, and Akira Masuda for technical advice for the in vivo electrophysiologic experiments. We thank Yuki Kobayashi for instruction on the viral purification, Kazuhiko Yamaguchi for critical comments, and all members of S.I.{\textquoteright}s laboratory for encouragement and help. The work was supported in part by Grant-in-Aid for Japan Society for the Promotion of Sciences (JSPS) Fellows (12J00301) to S.K., Grant-in-Aid for Young Scientists (B) from JSPS (25871135) to T.Y., the program for Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) from the Japan Agency for Medical Research and Development (AMED) to A.M. and S.I., the Ministry of Education, Culture, Sports, Science, and Technology (MEXT)-Supported Program for the Strategic Research Foundation at Private Universities, 2012–2016 to T.O., and Funding Program for World-Leading Innovative R&D on Science and Technology (FIRST Program) initiated by the Council for Science and Technology Policy (CSTP) to S.I. Publisher Copyright: {\textcopyright} 2018 The Author(s)",
year = "2018",
month = mar,
day = "13",
doi = "10.1016/j.celrep.2018.02.056",
language = "English",
volume = "22",
pages = "2873--2885",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "11",
}