Expression of rfamide-related peptide in the dorsomedial nucleus of hypothalamus during the estrous cycle of rats

Mohammad Reza Jafarzadeh Shirazi, Farid Pazhoohi, Mohammad Javad Zamiri, Mohammad Saied Salehi, Mohammad Reza Namavar, Amin Tamadon*, Nader Tanideh, Afsoon Zarei, Kazuyoshi Tsutsui

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)


    Introduction: RFamide-related peptide (RFRP) is believed to act as an inhibitor of gonadotropin releasing hormone (GnRH) secretion. The aim of the present study was to compare the expression pattern of RFRP neurons in the dorsomedial nucleus of hypothalamus (DMH) at different phases of the rat estrous cycle. Methods: The phases of the estrous cycle were determined in 16 adult female Sprague-Dawley rats using vaginal smears. The rats were divided into five groups: proestrus phase (n=4), early estrus phase (n=3), estrus phase (n=3), metestrus phase (n=3) and diestrus phase (n=3). After transcardial perfusion, their brains were removed and fixed. Diencephalon of each rat brain was sectioned, and DMH-containing sections were stained using an immunohistochemical method. The number of RFRP positive neurons in DMH was counted microscopically. Results: Almost all of the neurons expressing RFRP in the DMH were bipolar. Mean and standard error of the number of RFRP neurons during diestrus (45.8±12.6) and estrus phases (44.7±3.6) were greater (P<0.05) than early estrus (18.8±0.8) and proestrus phases (16.2±2.0). Conclusion: Results confirm a regulatory role for RFRP in DMH in the control of rat estrous cycle.

    Original languageEnglish
    Pages (from-to)72-79
    Number of pages8
    JournalPhysiology and Pharmacology
    Issue number1
    Publication statusPublished - 2013


    • Dorsomedial nucleus
    • Estrous cycle
    • Gonadotropin releasing hormone
    • Rat

    ASJC Scopus subject areas

    • Pharmacology
    • Physiology


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