Expression of the green fluorescent protein derivative S65T in Xenopus laevis oocytes

Julie M. Matheson; Atsushi Miyawaki; Akira Muto; Takafumi Inoue; Katsuhiko Mikoshiba

doi:10.2220/biomedres.17.221

Expression of the green fluorescent protein derivative S65T in Xenopus laevis oocytes

Julie M. Matheson, Atsushi Miyawaki, Akira Muto^*, Takafumi Inoue, Katsuhiko Mikoshiba

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The coding region of the green fluorescent protein mutant S65T has been fused to the eukaryotic expression vector pMT3 and injected into the nucleus of Xenopus laevis oocytes. The fluorescent gene product is observed within 24-48 h and can be used as an internal control to monitor gene expression without the need for added substrates. Co-injection of pMT3-S65T and a pMT3 construct containing the coding region of the mouse cerebellar type 1 inositol 1,4,5-trisphosphate receptor (IP₃R) showed that the fluorescence level of S65T paralleled expression of IP₃R. This correlation makes it possible to visually evaluate relative levels of IP₃R expression.

Original language	English
Pages (from-to)	221-225
Number of pages	5
Journal	Biomedical Research
Volume	17
Issue number	3
DOIs	https://doi.org/10.2220/biomedres.17.221
Publication status	Published - 1996 Jun
Externally published	Yes

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Access to Document

10.2220/biomedres.17.221

Cite this

@article{3ea7d56374074db3a269ce14c3d629e6,

title = "Expression of the green fluorescent protein derivative S65T in Xenopus laevis oocytes",

abstract = "The coding region of the green fluorescent protein mutant S65T has been fused to the eukaryotic expression vector pMT3 and injected into the nucleus of Xenopus laevis oocytes. The fluorescent gene product is observed within 24-48 h and can be used as an internal control to monitor gene expression without the need for added substrates. Co-injection of pMT3-S65T and a pMT3 construct containing the coding region of the mouse cerebellar type 1 inositol 1,4,5-trisphosphate receptor (IP3R) showed that the fluorescence level of S65T paralleled expression of IP3R. This correlation makes it possible to visually evaluate relative levels of IP3R expression.",

author = "Matheson, {Julie M.} and Atsushi Miyawaki and Akira Muto and Takafumi Inoue and Katsuhiko Mikoshiba",

year = "1996",

month = jun,

doi = "10.2220/biomedres.17.221",

language = "English",

volume = "17",

pages = "221--225",

journal = "Biomedical Research",

issn = "0388-6107",

publisher = "Biomedical Research Foundation",

number = "3",

}

TY - JOUR

T1 - Expression of the green fluorescent protein derivative S65T in Xenopus laevis oocytes

AU - Matheson, Julie M.

AU - Miyawaki, Atsushi

AU - Muto, Akira

AU - Inoue, Takafumi

AU - Mikoshiba, Katsuhiko

PY - 1996/6

Y1 - 1996/6

N2 - The coding region of the green fluorescent protein mutant S65T has been fused to the eukaryotic expression vector pMT3 and injected into the nucleus of Xenopus laevis oocytes. The fluorescent gene product is observed within 24-48 h and can be used as an internal control to monitor gene expression without the need for added substrates. Co-injection of pMT3-S65T and a pMT3 construct containing the coding region of the mouse cerebellar type 1 inositol 1,4,5-trisphosphate receptor (IP3R) showed that the fluorescence level of S65T paralleled expression of IP3R. This correlation makes it possible to visually evaluate relative levels of IP3R expression.

AB - The coding region of the green fluorescent protein mutant S65T has been fused to the eukaryotic expression vector pMT3 and injected into the nucleus of Xenopus laevis oocytes. The fluorescent gene product is observed within 24-48 h and can be used as an internal control to monitor gene expression without the need for added substrates. Co-injection of pMT3-S65T and a pMT3 construct containing the coding region of the mouse cerebellar type 1 inositol 1,4,5-trisphosphate receptor (IP3R) showed that the fluorescence level of S65T paralleled expression of IP3R. This correlation makes it possible to visually evaluate relative levels of IP3R expression.

UR - http://www.scopus.com/inward/record.url?scp=0029834333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029834333&partnerID=8YFLogxK

U2 - 10.2220/biomedres.17.221

DO - 10.2220/biomedres.17.221

M3 - Article

AN - SCOPUS:0029834333

SN - 0388-6107

VL - 17

SP - 221

EP - 225

JO - Biomedical Research

JF - Biomedical Research

IS - 3

ER -

Expression of the green fluorescent protein derivative S65T in Xenopus laevis oocytes

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this