TY - JOUR
T1 - Fabrication of high-aspect-ratio amorphous perfluorinated polymer structure for total internal reflection fluorescence microscopy
AU - Nakahara, Asahi
AU - Shirasaki, Yoshitaka
AU - Kawai, Kentaro
AU - Ohara, Osamu
AU - Mizuno, Jun
AU - Shoji, Shuichi
N1 - Funding Information:
This work was supported by the Special Postdoctoral Researchers Program from RIKEN and Waseda University Global COE Program “International Research and Education Center for Ambient SoC” sponsored by the Ministry of Education, Culture, Sports, Science and Technology, Japan.
PY - 2011/8
Y1 - 2011/8
N2 - A method to fabricate microstructures that are ideal for optical applications was developed. This method enabled structures with a high aspect ratio to be fabricated from an amorphous perfluorinated polymer, CYTOP, which has an identical refractive index to that of water. Using the method, a microwell array device was developed for the purpose of high-throughput protein quantification. Evaluation of the optical properties of the array using total internal reflection fluorescence (TIRF) microscopy demonstrated that the device did not interfere with TIRF imaging at the bottom of each microwell. This method can expand the possible applications of biophotonic devices as well as TIRF imaging.
AB - A method to fabricate microstructures that are ideal for optical applications was developed. This method enabled structures with a high aspect ratio to be fabricated from an amorphous perfluorinated polymer, CYTOP, which has an identical refractive index to that of water. Using the method, a microwell array device was developed for the purpose of high-throughput protein quantification. Evaluation of the optical properties of the array using total internal reflection fluorescence (TIRF) microscopy demonstrated that the device did not interfere with TIRF imaging at the bottom of each microwell. This method can expand the possible applications of biophotonic devices as well as TIRF imaging.
KW - Amorphous perfluorinated polymer
KW - High-aspect-ratio structure
KW - TIRF microscopy
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U2 - 10.1016/j.mee.2011.02.117
DO - 10.1016/j.mee.2011.02.117
M3 - Article
AN - SCOPUS:79960052542
SN - 0167-9317
VL - 88
SP - 1817
EP - 1820
JO - Microelectronic Engineering
JF - Microelectronic Engineering
IS - 8
ER -