Fabrication of micropatterned fish scale collagen scaffold using soft lithography for oral mucosa tissue engineering

Kazuma Kishimoto, Keito Miwa, Ayako Suzuki, Isamu Yamaguchi, Yoshihiro Kodama, Orakarn Suebsamarn, Shuichi Shoji, Kenji Izumi, Jun Mizuno

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Fish-derived collagen is considered a promising material for oral mucosa tissue engineering because it is free from risk of transmissible infectious diseases. We developed a novel fish scale collagen scaffold with micropatterns on which oral keratinocytes are cultured to create a tissue-engineered oral mucosa. Anisotropic and isotropic etching were used to fabricate negative patterns on the Si substrate and soft lithography was used to translate the patterns to PDMS and fish scale collagen. The histological evaluation of the tissue-engineered oral mucosa confirmed the successful formation of the epithelial layer mimicking oral mucosa in vivo. This study suggests that our novel approach for fabricating biomimetic scaffold is promising and will make a huge contribution to the field of oral mucosa regeneration.

Original languageEnglish
Title of host publication2021 International Conference on Electronics Packaging, ICEP 2021
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages25-26
Number of pages2
ISBN (Electronic)9784991191114
DOIs
Publication statusPublished - 2021 May 12
Event20th International Conference on Electronics Packaging, ICEP 2021 - Tokyo, Japan
Duration: 2021 May 122021 May 14

Publication series

Name2021 International Conference on Electronics Packaging, ICEP 2021

Conference

Conference20th International Conference on Electronics Packaging, ICEP 2021
Country/TerritoryJapan
CityTokyo
Period21/5/1221/5/14

Keywords

  • Biomimetics
  • Fish scale collagen
  • Oral mucosa
  • Scaffold
  • Soft lithography

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Mechanics of Materials
  • Electronic, Optical and Magnetic Materials
  • Instrumentation

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