Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting

Yumi Yamahara; Naotsugu Nagasawa; Mitsumasa Taguchi; Akihiro Oshima; Masakazu Washio

doi:10.1016/j.radphyschem.2017.02.045

Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting

Yumi Yamahara^*, Naotsugu Nagasawa, Mitsumasa Taguchi, Akihiro Oshima, Masakazu Washio

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy. It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20 °C. Water contact angle analysis indicated that obtained templates had thermo-response around 30 °C. It is suggested that the grafted polymer chains would mainly react with peroxy radicals (–CF₂–CF(OO･)–) on tetrafluoroethylene unit in ETFE.

Original language	English
Pages (from-to)	88-93
Number of pages	6
Journal	Radiation Physics and Chemistry
Volume	142
DOIs	https://doi.org/10.1016/j.radphyschem.2017.02.045
Publication status	Published - 2018 Jan

Keywords

Cell culture dishes
ETFE
PNIPPAm-g-ETFE
Pre-irradiation grafting
Thermo-responsive

ASJC Scopus subject areas

Radiation

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10.1016/j.radphyschem.2017.02.045

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@article{a6948793c0504765ad2062ef6106b0bb,

title = "Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting",

abstract = "Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy. It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20 °C. Water contact angle analysis indicated that obtained templates had thermo-response around 30 °C. It is suggested that the grafted polymer chains would mainly react with peroxy radicals (–CF2–CF(OO･)–) on tetrafluoroethylene unit in ETFE.",

keywords = "Cell culture dishes, ETFE, PNIPPAm-g-ETFE, Pre-irradiation grafting, Thermo-responsive",

author = "Yumi Yamahara and Naotsugu Nagasawa and Mitsumasa Taguchi and Akihiro Oshima and Masakazu Washio",

year = "2018",

month = jan,

doi = "10.1016/j.radphyschem.2017.02.045",

language = "English",

volume = "142",

pages = "88--93",

journal = "Radiation Physics and Chemistry",

issn = "0969-806X",

publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting

AU - Yamahara, Yumi

AU - Nagasawa, Naotsugu

AU - Taguchi, Mitsumasa

AU - Oshima, Akihiro

AU - Washio, Masakazu

PY - 2018/1

Y1 - 2018/1

N2 - Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy. It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20 °C. Water contact angle analysis indicated that obtained templates had thermo-response around 30 °C. It is suggested that the grafted polymer chains would mainly react with peroxy radicals (–CF2–CF(OO･)–) on tetrafluoroethylene unit in ETFE.

AB - Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy. It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20 °C. Water contact angle analysis indicated that obtained templates had thermo-response around 30 °C. It is suggested that the grafted polymer chains would mainly react with peroxy radicals (–CF2–CF(OO･)–) on tetrafluoroethylene unit in ETFE.

KW - Cell culture dishes

KW - ETFE

KW - PNIPPAm-g-ETFE

KW - Pre-irradiation grafting

KW - Thermo-responsive

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U2 - 10.1016/j.radphyschem.2017.02.045

DO - 10.1016/j.radphyschem.2017.02.045

M3 - Article

AN - SCOPUS:85014026055

SN - 0969-806X

VL - 142

SP - 88

EP - 93

JO - Radiation Physics and Chemistry

JF - Radiation Physics and Chemistry

ER -

Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting

Abstract

Keywords

ASJC Scopus subject areas

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