Facilely fabricated luminescent nanoparticle thermosensor for real-time microthermography in living animals

Ferdinandus, Satoshi Arai, Shinji Takeoka, Shinichi Ishiwata, Madoka Suzuki, Hirotaka Sato*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)


This paper presents a high-sensitivity luminescent nanoparticle thermosensor capable of real-time microthermography in a living organism. Microthermography, or microscopically visualizing the temperature distribution within living cells, tissues, and organisms, is a promising technology to explore various physiological activities at the microscale. Using a facile nanoprecipitation method, we fabricated a polymer-nanoparticle embedding EuDT, a thermosensitive high-luminescence-emitter dye molecule, and rhodamine 800, a luminescent molecule excitable with low energy light and less sensitive to temperature. The nanoparticle thermosensor was largely exempted from the background noise, which is the undesired luminescence from the target biological sample, enabling direct acquisition of luminescence intensities from the thermosensor within the specified area of 68 μm × 68 μm on the muscle tissue of a living insect, i.e., real-time microthermography, without the need of subtracting background noise. Thus, we successfully mapped out the temperature shift due to the animal's voluntary heat production. The nanoparticle thermosensor capable of in vivo temperature mapping must be a useful biological thermographic technology to explore microscopic heat productions in living organisms.

Original languageEnglish
Pages (from-to)1222-1227
Number of pages6
JournalACS Sensors
Issue number10
Publication statusPublished - 2016 Oct


  • Bioimaging
  • Fluorescent thermosensor
  • In vivo thermography
  • Luminescent thermosensor
  • Nanoparticle thermosensor
  • Temperature sensitive dye
  • Thermogenesis
  • Thermography

ASJC Scopus subject areas

  • Bioengineering
  • Instrumentation
  • Process Chemistry and Technology
  • Fluid Flow and Transfer Processes


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