Fast and sensitive bacteria detection by boronic acid modified fluorescent dendrimer

Ayame Mikagi; Riho Tsurufusa; Yuji Tsuchido; Takeshi Hashimoto; Takashi Hayashita

doi:10.3390/s21093115

Fast and sensitive bacteria detection by boronic acid modified fluorescent dendrimer

Ayame Mikagi, Riho Tsurufusa, Yuji Tsuchido, Takeshi Hashimoto, Takashi Hayashita^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

This study reports a novel, fast, easy, and sensitive detection method for bacteria which is urgently needed to diagnose infections in their early stages. Our work presents a complex of poly(amidoamine) dendrimer modified by phenylboronic acid and labeled by a fluorescent dansyl group (Dan-B8.5-PAMAM). Our system detects bacteria in 20 min with a sensitivity of approximately 10⁴ colony-forming units (CFU)·mL⁻¹ . Moreover, it does not require any peculiar technical skills or expensive materials. The driving force for bacteria recognition is the binding between terminal phenylboronic acids on the probe and bacteria’s surface glycolipids, rather than electrostatic interactions. The aggregation caused by such binding reduces fluorescence. Even though our recognition method does not distinguish between live or dead bacteria, it shows selective antibacterial activity towards Gram-negative bacteria. This study may potentially contribute a new method for the convenient detection and killing of bacteria.

Original language	English
Article number	3115
Journal	Sensors
Volume	21
Issue number	9
DOIs	https://doi.org/10.3390/s21093115
Publication status	Published - 2021 May 1

Keywords

Bacteria detection
Dendrimer
E. coli
Fluorescence measurement
Phenylboronic acid
S. aureus

ASJC Scopus subject areas

Analytical Chemistry
Information Systems
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering

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10.3390/s21093115

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title = "Fast and sensitive bacteria detection by boronic acid modified fluorescent dendrimer",

abstract = "This study reports a novel, fast, easy, and sensitive detection method for bacteria which is urgently needed to diagnose infections in their early stages. Our work presents a complex of poly(amidoamine) dendrimer modified by phenylboronic acid and labeled by a fluorescent dansyl group (Dan-B8.5-PAMAM). Our system detects bacteria in 20 min with a sensitivity of approximately 104 colony-forming units (CFU)·mL−1 . Moreover, it does not require any peculiar technical skills or expensive materials. The driving force for bacteria recognition is the binding between terminal phenylboronic acids on the probe and bacteria{\textquoteright}s surface glycolipids, rather than electrostatic interactions. The aggregation caused by such binding reduces fluorescence. Even though our recognition method does not distinguish between live or dead bacteria, it shows selective antibacterial activity towards Gram-negative bacteria. This study may potentially contribute a new method for the convenient detection and killing of bacteria.",

keywords = "Bacteria detection, Dendrimer, E. coli, Fluorescence measurement, Phenylboronic acid, S. aureus",

author = "Ayame Mikagi and Riho Tsurufusa and Yuji Tsuchido and Takeshi Hashimoto and Takashi Hayashita",

note = "Funding Information: Acknowledgments: A JSPS Research Fellowship for Young Scientists (DC), awarded to A.M., is gratefully acknowledged. Funding Information: Funding: This work was financially supported by a Grant-in-Aid for JSPS Fellows (Grant No. 20J20891), a Grant-in-Aid for Early-Career Scientists (Grant No. 18K14255), a Grant-in Aid for Scientific Research (C) (Grant No. 18K05180) and a Grant-in Aid for Scientific Research (B) (Grant No. 20H02772) from the Japan Society for the Promotion of Science (JSPS). Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

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doi = "10.3390/s21093115",

language = "English",

volume = "21",

journal = "Sensors",

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T1 - Fast and sensitive bacteria detection by boronic acid modified fluorescent dendrimer

AU - Mikagi, Ayame

AU - Tsurufusa, Riho

AU - Tsuchido, Yuji

AU - Hashimoto, Takeshi

AU - Hayashita, Takashi

N1 - Funding Information: Acknowledgments: A JSPS Research Fellowship for Young Scientists (DC), awarded to A.M., is gratefully acknowledged. Funding Information: Funding: This work was financially supported by a Grant-in-Aid for JSPS Fellows (Grant No. 20J20891), a Grant-in-Aid for Early-Career Scientists (Grant No. 18K14255), a Grant-in Aid for Scientific Research (C) (Grant No. 18K05180) and a Grant-in Aid for Scientific Research (B) (Grant No. 20H02772) from the Japan Society for the Promotion of Science (JSPS). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/5/1

Y1 - 2021/5/1

N2 - This study reports a novel, fast, easy, and sensitive detection method for bacteria which is urgently needed to diagnose infections in their early stages. Our work presents a complex of poly(amidoamine) dendrimer modified by phenylboronic acid and labeled by a fluorescent dansyl group (Dan-B8.5-PAMAM). Our system detects bacteria in 20 min with a sensitivity of approximately 104 colony-forming units (CFU)·mL−1 . Moreover, it does not require any peculiar technical skills or expensive materials. The driving force for bacteria recognition is the binding between terminal phenylboronic acids on the probe and bacteria’s surface glycolipids, rather than electrostatic interactions. The aggregation caused by such binding reduces fluorescence. Even though our recognition method does not distinguish between live or dead bacteria, it shows selective antibacterial activity towards Gram-negative bacteria. This study may potentially contribute a new method for the convenient detection and killing of bacteria.

AB - This study reports a novel, fast, easy, and sensitive detection method for bacteria which is urgently needed to diagnose infections in their early stages. Our work presents a complex of poly(amidoamine) dendrimer modified by phenylboronic acid and labeled by a fluorescent dansyl group (Dan-B8.5-PAMAM). Our system detects bacteria in 20 min with a sensitivity of approximately 104 colony-forming units (CFU)·mL−1 . Moreover, it does not require any peculiar technical skills or expensive materials. The driving force for bacteria recognition is the binding between terminal phenylboronic acids on the probe and bacteria’s surface glycolipids, rather than electrostatic interactions. The aggregation caused by such binding reduces fluorescence. Even though our recognition method does not distinguish between live or dead bacteria, it shows selective antibacterial activity towards Gram-negative bacteria. This study may potentially contribute a new method for the convenient detection and killing of bacteria.

KW - Bacteria detection

KW - Dendrimer

KW - E. coli

KW - Fluorescence measurement

KW - Phenylboronic acid

KW - S. aureus

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Fast and sensitive bacteria detection by boronic acid modified fluorescent dendrimer

Abstract

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