Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system

Muneki Isokawa; Yanting Song; Katsuya Takatsuki; Tetsushi Sekiguchi; Jun Mizuno; Takashi Funatsu; Shuichi Shoji; Makoto Tsunoda

Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system

Muneki Isokawa, Yanting Song, Katsuya Takatsuki, Tetsushi Sekiguchi, Jun Mizuno, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda^*

^*Corresponding author for this work

Research Organization for Nano & Life Innovation

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A rapid and quantitative method for the determination of phenylalanine (Phe) and tyrosine (Tyr) in plasma using pressure-driven liquid chromatography (LC) on a chip was developed. After derivatizing Phe and Tyr with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), separation was performed within 140 s on a previously developed integration chip with a pillar array column and a gradient elution system [1,2]. The Phe and Tyr in plasma were quantified and good validation data were obtained. As Phe and Tyr are considered to be important biomarkers of some diseases, especially for newborn screening, the developed method is promising for rapid diagnosis in the clinical field.

Original language	English
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Publisher	Chemical and Biological Microsystems Society
Pages	941-943
Number of pages	3
ISBN (Print)	9780979806476
Publication status	Published - 2014
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 2014 Oct 26 → 2014 Oct 30

Other

Other	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/Territory	United States
City	San Antonio
Period	14/10/26 → 14/10/30

Keywords

Amino acids
Fluorescence
Liquid chromatography
Quantitative determination

ASJC Scopus subject areas

Control and Systems Engineering

Cite this

Isokawa, M., Song, Y., Takatsuki, K., Sekiguchi, T., Mizuno, J., Funatsu, T., Shoji, S., & Tsunoda, M. (2014). Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 941-943). Chemical and Biological Microsystems Society.

Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system. / Isokawa, Muneki; Song, Yanting; Takatsuki, Katsuya et al.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 941-943.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Isokawa, M, Song, Y, Takatsuki, K, Sekiguchi, T, Mizuno, J, Funatsu, T, Shoji, S & Tsunoda, M 2014, Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 941-943, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 14/10/26.

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abstract = "A rapid and quantitative method for the determination of phenylalanine (Phe) and tyrosine (Tyr) in plasma using pressure-driven liquid chromatography (LC) on a chip was developed. After derivatizing Phe and Tyr with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), separation was performed within 140 s on a previously developed integration chip with a pillar array column and a gradient elution system [1,2]. The Phe and Tyr in plasma were quantified and good validation data were obtained. As Phe and Tyr are considered to be important biomarkers of some diseases, especially for newborn screening, the developed method is promising for rapid diagnosis in the clinical field.",

keywords = "Amino acids, Fluorescence, Liquid chromatography, Quantitative determination",

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AU - Isokawa, Muneki

AU - Song, Yanting

AU - Takatsuki, Katsuya

AU - Sekiguchi, Tetsushi

AU - Mizuno, Jun

AU - Funatsu, Takashi

AU - Shoji, Shuichi

AU - Tsunoda, Makoto

PY - 2014

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N2 - A rapid and quantitative method for the determination of phenylalanine (Phe) and tyrosine (Tyr) in plasma using pressure-driven liquid chromatography (LC) on a chip was developed. After derivatizing Phe and Tyr with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), separation was performed within 140 s on a previously developed integration chip with a pillar array column and a gradient elution system [1,2]. The Phe and Tyr in plasma were quantified and good validation data were obtained. As Phe and Tyr are considered to be important biomarkers of some diseases, especially for newborn screening, the developed method is promising for rapid diagnosis in the clinical field.

AB - A rapid and quantitative method for the determination of phenylalanine (Phe) and tyrosine (Tyr) in plasma using pressure-driven liquid chromatography (LC) on a chip was developed. After derivatizing Phe and Tyr with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), separation was performed within 140 s on a previously developed integration chip with a pillar array column and a gradient elution system [1,2]. The Phe and Tyr in plasma were quantified and good validation data were obtained. As Phe and Tyr are considered to be important biomarkers of some diseases, especially for newborn screening, the developed method is promising for rapid diagnosis in the clinical field.

KW - Amino acids

KW - Fluorescence

KW - Liquid chromatography

KW - Quantitative determination

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Fast and simultaneous analysis of phenylalanine and tyrosine in plasma using pillar array columns with a gradient elution system

Abstract

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Keywords

ASJC Scopus subject areas

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