FGF-2/calcium phosphate composite layer to resist bacterial infection

Y. Sogo; A. Ito; H. Mutsuzaki; A. Oyane; M. Onoguchi; N. Ichinose; M. Sakane; N. Ochiai

doi:10.4028/0-87849-422-7.691

FGF-2/calcium phosphate composite layer to resist bacterial infection

Y. Sogo^*, A. Ito, H. Mutsuzaki, A. Oyane, M. Onoguchi, N. Ichinose, M. Sakane, N. Ochiai

^*Corresponding author for this work

Waseda Research Institute for Science and Engineering

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

A FGF-2-apatite composite layer (FGF-AP layer) was formed on the surface of Ti screws in a supersaturated calcium phosphate solution supplemented with FGF-2. By an in vitro study using fibroblastic NIH3T3 cells, it was confirmed that FGF-2 was immobilized in the layer without complete denaturation although the composite layer was formed at 37°C. When Ti screws with the FGF-AP layer were percutaneously implanted in the proximal tibial metaphysis of 16 rabbits, no osteomyelitis was observed in any rabbits although a FGF-2-free AP layer allowed osteomyelitis in some cases in our previous study. These results suggest that a FGF-AP layer formed on Ti screws is useful for resisting bacterial infection during external fixations.

Original language	English
Pages (from-to)	691-694
Number of pages	4
Journal	Key Engineering Materials
Volume	330-332 I
DOIs	https://doi.org/10.4028/0-87849-422-7.691
Publication status	Published - 2007

Keywords

Adhesion
Bacterial infection
Coprecipitation
External fixation
FGF-2

ASJC Scopus subject areas

Materials Science(all)
Mechanics of Materials
Mechanical Engineering

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title = "FGF-2/calcium phosphate composite layer to resist bacterial infection",

abstract = "A FGF-2-apatite composite layer (FGF-AP layer) was formed on the surface of Ti screws in a supersaturated calcium phosphate solution supplemented with FGF-2. By an in vitro study using fibroblastic NIH3T3 cells, it was confirmed that FGF-2 was immobilized in the layer without complete denaturation although the composite layer was formed at 37°C. When Ti screws with the FGF-AP layer were percutaneously implanted in the proximal tibial metaphysis of 16 rabbits, no osteomyelitis was observed in any rabbits although a FGF-2-free AP layer allowed osteomyelitis in some cases in our previous study. These results suggest that a FGF-AP layer formed on Ti screws is useful for resisting bacterial infection during external fixations.",

keywords = "Adhesion, Bacterial infection, Coprecipitation, External fixation, FGF-2",

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doi = "10.4028/0-87849-422-7.691",

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volume = "330-332 I",

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T1 - FGF-2/calcium phosphate composite layer to resist bacterial infection

AU - Sogo, Y.

AU - Ito, A.

AU - Mutsuzaki, H.

AU - Oyane, A.

AU - Onoguchi, M.

AU - Ichinose, N.

AU - Sakane, M.

AU - Ochiai, N.

PY - 2007

Y1 - 2007

N2 - A FGF-2-apatite composite layer (FGF-AP layer) was formed on the surface of Ti screws in a supersaturated calcium phosphate solution supplemented with FGF-2. By an in vitro study using fibroblastic NIH3T3 cells, it was confirmed that FGF-2 was immobilized in the layer without complete denaturation although the composite layer was formed at 37°C. When Ti screws with the FGF-AP layer were percutaneously implanted in the proximal tibial metaphysis of 16 rabbits, no osteomyelitis was observed in any rabbits although a FGF-2-free AP layer allowed osteomyelitis in some cases in our previous study. These results suggest that a FGF-AP layer formed on Ti screws is useful for resisting bacterial infection during external fixations.

AB - A FGF-2-apatite composite layer (FGF-AP layer) was formed on the surface of Ti screws in a supersaturated calcium phosphate solution supplemented with FGF-2. By an in vitro study using fibroblastic NIH3T3 cells, it was confirmed that FGF-2 was immobilized in the layer without complete denaturation although the composite layer was formed at 37°C. When Ti screws with the FGF-AP layer were percutaneously implanted in the proximal tibial metaphysis of 16 rabbits, no osteomyelitis was observed in any rabbits although a FGF-2-free AP layer allowed osteomyelitis in some cases in our previous study. These results suggest that a FGF-AP layer formed on Ti screws is useful for resisting bacterial infection during external fixations.

KW - Adhesion

KW - Bacterial infection

KW - Coprecipitation

KW - External fixation

KW - FGF-2

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SP - 691

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JO - Key Engineering Materials

JF - Key Engineering Materials

ER -

FGF-2/calcium phosphate composite layer to resist bacterial infection

Abstract

Keywords

ASJC Scopus subject areas

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