FGF-4 regulates neural progenitor cell proliferation and neuronal differentiation

Nobuyoshi Kosaka, Maho Kodama, Hideo Sasaki, Yusuke Yamamoto, Fumitaka Takeshita, Yasushi Takahama, Hiromi Sakamoto, Takashi Kato, Masaaki Terada, Takahiro Ochiya*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)


The FGF-4 (fibroblast growth factor 4, known as HST-1) protein is an important mitogen for a variety of cell types. However, only limited information is available concerning tissue distribution and the biological role of FGF-4 in the brain. In situ hybridization analysis revealed localization of mouse Fgf-4 mRNA in the normal postnatal mouse hippocampus, subventricular zone (SVZ), and the rostral migratory stream where new neurons generate, migrate, and become incorporated into the functional circuitry of the brain. We also investigated whether FGF-4 could promote both proliferation and differentiation of the neural progenitor cells by using an in vitro neurosphere assay. The addition of recombinant FGF-4 generated large proliferative spheres that have a multipotent differentiation ability. Furthermore, recombinant FGF-4 significantly promotes neuronal differentiation in attached clonal neurosphere culture. These findings suggest that FGF-4 has an ability to promote neural stem cell proliferation and neuronal differentiation in the postnatal brain.

Original languageEnglish
Pages (from-to)E623-E629
JournalFASEB Journal
Issue number9
Publication statusPublished - 2006 Jul


  • FGF-4
  • Neural progenitor cell
  • Neurogenesis
  • Self-renewal

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics


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