Flow immunoassay using a cation exchange column and fluorescence-labelled antibody for detection of allergen

Tae Kyu Lim; Noriyuki Nakamura; Tadashi Matsunaga

doi:10.1016/S0003-2670(97)00438-8

Flow immunoassay using a cation exchange column and fluorescence-labelled antibody for detection of allergen

Tae Kyu Lim, Noriyuki Nakamura, Tadashi Matsunaga^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

An allergen detection was carried out based on a fluorescence immunoassay with a flow injection system. Allergen and fluorescein isothiocyanate (FITC) conjugated IgE antibody complex was separated from free FITC conjugated IgE antibody using a cation exchange column. The fluorescence intensity correlated linearly with a concentration of allergen in the range of 0.01-2.0 mg ml^-1. Reuse of free FITC conjugated IgE was possible. This simple and rapid immunoassay method can be used for the continuous detection of allergen.

Original language	English
Pages (from-to)	29-34
Number of pages	6
Journal	Analytica Chimica Acta
Volume	354
Issue number	1-3
DOIs	https://doi.org/10.1016/S0003-2670(97)00438-8
Publication status	Published - 1997 Nov 10
Externally published	Yes

Keywords

Allergen detection
Antibody
Cation exchange column
Flow injection
Fluorescence
Immunoassay

ASJC Scopus subject areas

Biochemistry
Analytical Chemistry
Spectroscopy
Environmental Chemistry

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10.1016/S0003-2670(97)00438-8

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title = "Flow immunoassay using a cation exchange column and fluorescence-labelled antibody for detection of allergen",

abstract = "An allergen detection was carried out based on a fluorescence immunoassay with a flow injection system. Allergen and fluorescein isothiocyanate (FITC) conjugated IgE antibody complex was separated from free FITC conjugated IgE antibody using a cation exchange column. The fluorescence intensity correlated linearly with a concentration of allergen in the range of 0.01-2.0 mg ml-1. Reuse of free FITC conjugated IgE was possible. This simple and rapid immunoassay method can be used for the continuous detection of allergen.",

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author = "Lim, {Tae Kyu} and Noriyuki Nakamura and Tadashi Matsunaga",

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T1 - Flow immunoassay using a cation exchange column and fluorescence-labelled antibody for detection of allergen

AU - Lim, Tae Kyu

AU - Nakamura, Noriyuki

AU - Matsunaga, Tadashi

PY - 1997/11/10

Y1 - 1997/11/10

N2 - An allergen detection was carried out based on a fluorescence immunoassay with a flow injection system. Allergen and fluorescein isothiocyanate (FITC) conjugated IgE antibody complex was separated from free FITC conjugated IgE antibody using a cation exchange column. The fluorescence intensity correlated linearly with a concentration of allergen in the range of 0.01-2.0 mg ml-1. Reuse of free FITC conjugated IgE was possible. This simple and rapid immunoassay method can be used for the continuous detection of allergen.

AB - An allergen detection was carried out based on a fluorescence immunoassay with a flow injection system. Allergen and fluorescein isothiocyanate (FITC) conjugated IgE antibody complex was separated from free FITC conjugated IgE antibody using a cation exchange column. The fluorescence intensity correlated linearly with a concentration of allergen in the range of 0.01-2.0 mg ml-1. Reuse of free FITC conjugated IgE was possible. This simple and rapid immunoassay method can be used for the continuous detection of allergen.

KW - Allergen detection

KW - Antibody

KW - Cation exchange column

KW - Flow injection

KW - Fluorescence

KW - Immunoassay

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M3 - Article

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SN - 0003-2670

VL - 354

SP - 29

EP - 34

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1-3

ER -

Flow immunoassay using a cation exchange column and fluorescence-labelled antibody for detection of allergen

Abstract

Keywords

ASJC Scopus subject areas

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