Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription

Kazuhiro Furukawa; Hiroshi Abe; Naoko Abe; Mitsuru Harada; Satoshi Tsuneda; Yoshihiro Ito

doi:10.1016/j.bmcl.2008.07.040

Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription

Kazuhiro Furukawa, Hiroshi Abe^*, Naoko Abe, Mitsuru Harada, Satoshi Tsuneda, Yoshihiro Ito

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

We demonstrate a generation of tandem repeats of a malachite green (MG) RNA aptamer using rolling circle transcription. To keep the higher-order structure of each aptamer on long RNA, we designed a sequence of circular DNA with a 14-base linker. T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA aptamer. Finally, the generation of the fluorescence signal was confirmed from aptamer repeats with MG.

Original language	English
Pages (from-to)	4562-4565
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	18
Issue number	16
DOIs	https://doi.org/10.1016/j.bmcl.2008.07.040
Publication status	Published - 2008 Aug 15

Keywords

Amplification
Fluorescence
Nucleic acid
Polymerase
RNA

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2008.07.040

Cite this

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title = "Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription",

abstract = "We demonstrate a generation of tandem repeats of a malachite green (MG) RNA aptamer using rolling circle transcription. To keep the higher-order structure of each aptamer on long RNA, we designed a sequence of circular DNA with a 14-base linker. T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA aptamer. Finally, the generation of the fluorescence signal was confirmed from aptamer repeats with MG.",

keywords = "Amplification, Fluorescence, Nucleic acid, Polymerase, RNA",

author = "Kazuhiro Furukawa and Hiroshi Abe and Naoko Abe and Mitsuru Harada and Satoshi Tsuneda and Yoshihiro Ito",

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TY - JOUR

T1 - Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription

AU - Furukawa, Kazuhiro

AU - Abe, Hiroshi

AU - Abe, Naoko

AU - Harada, Mitsuru

AU - Tsuneda, Satoshi

AU - Ito, Yoshihiro

N1 - Funding Information: H.A. was financially supported by the Riken Directors’ Fund, MEXT (Ministry of Education, Culture, Sports, Science, and Technology), and NEDO (New Energy and Industrial Technology Development Organization).

PY - 2008/8/15

Y1 - 2008/8/15

N2 - We demonstrate a generation of tandem repeats of a malachite green (MG) RNA aptamer using rolling circle transcription. To keep the higher-order structure of each aptamer on long RNA, we designed a sequence of circular DNA with a 14-base linker. T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA aptamer. Finally, the generation of the fluorescence signal was confirmed from aptamer repeats with MG.

AB - We demonstrate a generation of tandem repeats of a malachite green (MG) RNA aptamer using rolling circle transcription. To keep the higher-order structure of each aptamer on long RNA, we designed a sequence of circular DNA with a 14-base linker. T7 RNA polymerase was superior to Escherichia coli RNA polymerase in the specific transcription of the MG RNA aptamer. Finally, the generation of the fluorescence signal was confirmed from aptamer repeats with MG.

KW - Amplification

KW - Fluorescence

KW - Nucleic acid

KW - Polymerase

KW - RNA

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M3 - Article

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AN - SCOPUS:48649097165

SN - 0960-894X

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EP - 4565

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 16

ER -

Fluorescence generation from tandem repeats of a malachite green RNA aptamer using rolling circle transcription

Abstract

Keywords

ASJC Scopus subject areas

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