@article{adb97fa252e2496cba11f5b2278878d7,
title = "Fluorescence laser microdissection reveals a distinct pattern of gene activation in the mouse hippocampal region",
abstract = "A histoanatomical context is imperative in an analysis of gene expression in a cell in a tissue to elucidate physiological function of the cell. In this study, we made technical advances in fluorescence laser microdissection (LMD) in combination with the absolute quantification of small amounts of mRNAs from a region of interest (ROI) in fluorescence-labeled tissue sections. We demonstrate that our fluorescence LMD-RTqPCR method has three orders of dynamic range, with the lower limit of ROI-size corresponding to a single cell. The absolute quantification of the expression levels of the immediate early genes in an ROI equivalent to a few hundred neurons in the hippocampus revealed that mice transferred from their home cage to a novel environment have distinct activation profiles in the hippocampal regions (CA1, CA3, and DG) and that the gene expression pattern in CA1, but not in the other regions, follows a power law distribution.",
author = "Wataru Yoshioka and Nozomi Endo and Akie Kurashige and Asahi Haijima and Toshihiro Endo and Toshiyuki Shibata and Ryutaro Nishiyama and Masaki Kakeyama and Chiharu Tohyama",
note = "Funding Information: This work was supported by the research project of the Cooperative Research of Leica-Microsystems and the University of Tokyo to M.K., and the Strategic Research Program for Brain Sciences (SRPBS) of the Ministry of Education, Culture, Sports, Science and Technology to C.T., Grant-in-Aid to M.K. and C.T. from the Japan Society for the Promotion of Science. R.N. is an employee of Leica Microsystems. We thank Dr. K. Nakajima of Keio University for his kind guidance concerning the in utero electroporation technique, Drs. S. Shibata of Waseda University and C. Watanabe of the University of Tokyo, K. Tani and Y. Takahashi of Tokyo University of Pharmacy and Life Sciences for their helpful discussions, and A. Shimazaki and Y. Hirasawa for their excellent technical assistance.",
year = "2012",
doi = "10.1038/srep00783",
language = "English",
volume = "2",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}