Fluorescence microscopic investigation on morphological changes of giant multilamellar vesicles induced by amphiphilic additives

Taro Toyota; Hirotatsu Tsuha; Koji Yamada; Katsuto Takakura; Kenji Yasuda; Tadashi Sugawara

doi:10.1021/la0529198

Fluorescence microscopic investigation on morphological changes of giant multilamellar vesicles induced by amphiphilic additives

Taro Toyota, Hirotatsu Tsuha, Koji Yamada, Katsuto Takakura, Kenji Yasuda, Tadashi Sugawara^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Adding an artificial bolaamphiphile to a dispersion of giant multilamellar vesicles (GMVs) made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) induced a cup-shaped deformation in GMVs accompanied by partial extrusion of the inner vesicle; thereafter, the deformed vesicles returned to their original shape. On the other hand, when the artificial bolaamphiphile together with a surfactant was added to the vesicular dispersion, these deformation and reformation dynamics were transmitted from the outer membranes in GMVs to the inner membranes until an intact inner vesicle was extruded out of the outer membrane. The microscopic aspects of these processes were investigated using amphiphiles tagged with individual fluorophores.

Original language	English
Pages (from-to)	1976-1981
Number of pages	6
Journal	Langmuir
Volume	22
Issue number	5
DOIs	https://doi.org/10.1021/la0529198
Publication status	Published - 2006 Feb 28
Externally published	Yes

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Surfaces and Interfaces
Spectroscopy
Electrochemistry

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abstract = "Adding an artificial bolaamphiphile to a dispersion of giant multilamellar vesicles (GMVs) made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) induced a cup-shaped deformation in GMVs accompanied by partial extrusion of the inner vesicle; thereafter, the deformed vesicles returned to their original shape. On the other hand, when the artificial bolaamphiphile together with a surfactant was added to the vesicular dispersion, these deformation and reformation dynamics were transmitted from the outer membranes in GMVs to the inner membranes until an intact inner vesicle was extruded out of the outer membrane. The microscopic aspects of these processes were investigated using amphiphiles tagged with individual fluorophores.",

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T1 - Fluorescence microscopic investigation on morphological changes of giant multilamellar vesicles induced by amphiphilic additives

AU - Toyota, Taro

AU - Tsuha, Hirotatsu

AU - Yamada, Koji

AU - Takakura, Katsuto

AU - Yasuda, Kenji

AU - Sugawara, Tadashi

PY - 2006/2/28

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AB - Adding an artificial bolaamphiphile to a dispersion of giant multilamellar vesicles (GMVs) made of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine (POPC) induced a cup-shaped deformation in GMVs accompanied by partial extrusion of the inner vesicle; thereafter, the deformed vesicles returned to their original shape. On the other hand, when the artificial bolaamphiphile together with a surfactant was added to the vesicular dispersion, these deformation and reformation dynamics were transmitted from the outer membranes in GMVs to the inner membranes until an intact inner vesicle was extruded out of the outer membrane. The microscopic aspects of these processes were investigated using amphiphiles tagged with individual fluorophores.

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Fluorescence microscopic investigation on morphological changes of giant multilamellar vesicles induced by amphiphilic additives

Abstract

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