Foreign gene expression in marine cyanobacteria under pseudo-continuous culture

Koji Sode; Masahiro Tatara; Naoaki Hatano; Tadashi Matsunaga

doi:10.1016/0168-1656(94)90072-8

Foreign gene expression in marine cyanobacteria under pseudo-continuous culture

Koji Sode^*, Masahiro Tatara, Naoaki Hatano, Tadashi Matsunaga

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Foreign gene expression in a marine cyanobacterium, Synechococcus NKBG 15041c, has been carried out in both batch and pseudo-continuous culture, using chloramphenicol acetyltransferase (CAT) as a model peptide and the broad host range vector pKT230. CAT has been successfully expressed in marine cyanobacteria under kanamycin resistance gene promoter. Pseudo-continuous culture of these recombinant marine cyanobacteria has been done by varying the dilution rate. At a dilution rate between 0.01 to 0.02 h^-1, highest productivity was achieved. Under these conditions, long-term pseudo-continuous culture of recombinant marine cyanobacteria was achieved for more than 600 h with CAT productivity 18-fold greater than that observed in batch culture.

Original language	English
Pages (from-to)	243-248
Number of pages	6
Journal	Journal of Biotechnology
Volume	33
Issue number	3
DOIs	https://doi.org/10.1016/0168-1656(94)90072-8
Publication status	Published - 1994 Apr 15
Externally published	Yes

Keywords

Broad host range vector
Chloramphenicol acetyl-transferase
Foreign gene expression
Marine cyanobacteria
Pseudo-continuous culture

ASJC Scopus subject areas

Biotechnology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0168-1656(94)90072-8

Cite this

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title = "Foreign gene expression in marine cyanobacteria under pseudo-continuous culture",

abstract = "Foreign gene expression in a marine cyanobacterium, Synechococcus NKBG 15041c, has been carried out in both batch and pseudo-continuous culture, using chloramphenicol acetyltransferase (CAT) as a model peptide and the broad host range vector pKT230. CAT has been successfully expressed in marine cyanobacteria under kanamycin resistance gene promoter. Pseudo-continuous culture of these recombinant marine cyanobacteria has been done by varying the dilution rate. At a dilution rate between 0.01 to 0.02 h-1, highest productivity was achieved. Under these conditions, long-term pseudo-continuous culture of recombinant marine cyanobacteria was achieved for more than 600 h with CAT productivity 18-fold greater than that observed in batch culture.",

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T1 - Foreign gene expression in marine cyanobacteria under pseudo-continuous culture

AU - Sode, Koji

AU - Tatara, Masahiro

AU - Hatano, Naoaki

AU - Matsunaga, Tadashi

PY - 1994/4/15

Y1 - 1994/4/15

N2 - Foreign gene expression in a marine cyanobacterium, Synechococcus NKBG 15041c, has been carried out in both batch and pseudo-continuous culture, using chloramphenicol acetyltransferase (CAT) as a model peptide and the broad host range vector pKT230. CAT has been successfully expressed in marine cyanobacteria under kanamycin resistance gene promoter. Pseudo-continuous culture of these recombinant marine cyanobacteria has been done by varying the dilution rate. At a dilution rate between 0.01 to 0.02 h-1, highest productivity was achieved. Under these conditions, long-term pseudo-continuous culture of recombinant marine cyanobacteria was achieved for more than 600 h with CAT productivity 18-fold greater than that observed in batch culture.

AB - Foreign gene expression in a marine cyanobacterium, Synechococcus NKBG 15041c, has been carried out in both batch and pseudo-continuous culture, using chloramphenicol acetyltransferase (CAT) as a model peptide and the broad host range vector pKT230. CAT has been successfully expressed in marine cyanobacteria under kanamycin resistance gene promoter. Pseudo-continuous culture of these recombinant marine cyanobacteria has been done by varying the dilution rate. At a dilution rate between 0.01 to 0.02 h-1, highest productivity was achieved. Under these conditions, long-term pseudo-continuous culture of recombinant marine cyanobacteria was achieved for more than 600 h with CAT productivity 18-fold greater than that observed in batch culture.

KW - Broad host range vector

KW - Chloramphenicol acetyl-transferase

KW - Foreign gene expression

KW - Marine cyanobacteria

KW - Pseudo-continuous culture

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Foreign gene expression in marine cyanobacteria under pseudo-continuous culture

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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