TY - JOUR
T1 - Functional evaluation of hemoglobin- and lipidheme-vesicles as red cell substitutes
AU - Sakai, Hiromi
AU - Hamada, Kenichi
AU - Takeoka, Shinji
AU - Nishide, Hiroyuki
AU - Tsuchida, Eishun
PY - 1996/8
Y1 - 1996/8
N2 - The two kinds of red cell substitutes, hemoglobin-vesicles (HbV) and lipidheme-vesicles (LihV, totally synthetic oxygen carrier), were evaluated in terms of physicochemical properties such as binding and dissociating reactions of ligands (CO, O2 and NO), rheological and structural properties. Carbonylation of Hb during the purification of Hb and the preparation of HbV is effective to prevent Hb denaturation. The rates of oxygenation of both HbV and LihV are faster than that of red blood cells (RBC). Their oxygen affinities (P50, HbV, 32 mmHg; LihV, 43 mmHg, cf. RBC, 28 mmHg) can be controlled to transport a sufficient amount of oxygen comparable with that of RBC. The smaller sizes of vesicles are advantageous for prompt ligand reaction and low viscosity. Both HbV and RBC show about 110 times less vasoconstrictive effects than stripped Hb. HbV shows only one sixth of the slow binding rate of NO (=endothelial derived relaxation factor) in comparison with stripped Hb. Inhibition of vasoconstriction by those vesicles is discussed from the kinetic data.
AB - The two kinds of red cell substitutes, hemoglobin-vesicles (HbV) and lipidheme-vesicles (LihV, totally synthetic oxygen carrier), were evaluated in terms of physicochemical properties such as binding and dissociating reactions of ligands (CO, O2 and NO), rheological and structural properties. Carbonylation of Hb during the purification of Hb and the preparation of HbV is effective to prevent Hb denaturation. The rates of oxygenation of both HbV and LihV are faster than that of red blood cells (RBC). Their oxygen affinities (P50, HbV, 32 mmHg; LihV, 43 mmHg, cf. RBC, 28 mmHg) can be controlled to transport a sufficient amount of oxygen comparable with that of RBC. The smaller sizes of vesicles are advantageous for prompt ligand reaction and low viscosity. Both HbV and RBC show about 110 times less vasoconstrictive effects than stripped Hb. HbV shows only one sixth of the slow binding rate of NO (=endothelial derived relaxation factor) in comparison with stripped Hb. Inhibition of vasoconstriction by those vesicles is discussed from the kinetic data.
KW - Hemoglobin-vesicles
KW - Lipidheme-vesicles
KW - Nitric oxide
KW - Oxygen carrier
KW - Red cell substitutes
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U2 - 10.1002/(SICI)1099-1581(199608)7:8<639::AID-PAT572>3.0.CO;2-2
DO - 10.1002/(SICI)1099-1581(199608)7:8<639::AID-PAT572>3.0.CO;2-2
M3 - Review article
AN - SCOPUS:0030217741
SN - 1042-7147
VL - 7
SP - 639
EP - 644
JO - Polymers for Advanced Technologies
JF - Polymers for Advanced Technologies
IS - 8
ER -