Generation of phenoxy radicals by methemoglobin-hydrogen peroxide studied by electron paramagnetic resonance

Takeshi Shiga*, Kazuhiko Imaizumi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The free radical intermediates of phenol derivatives, produced by the methemoglobin-hydrogen peroxide system at pH 5 and 7, are detected by electron paramagnetic resonance equipped with a continuous-flow apparatus. All the radicals from phenols are the phenoxy radicals, as identified by analyzing the observed hyperfine structures of the spectra with the aid of SCF-LCAO MO calculations. Comparing with the reaction of Fenton's reagent, it is concluded that free OH radical, even if it exists, is not liberated into the solution in the methemoglobin-hydrogen peroxide system.

Original languageEnglish
Pages (from-to)540-547
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume154
Issue number2
DOIs
Publication statusPublished - 1973
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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