TY - JOUR
T1 - Genetically engineered biosynthetic pathways for nonnatural C 60 carotenoids using C 5 -elongases and C 50 -cyclases in Escherichia coli
AU - Li, Ling
AU - Furubayashi, Maiko
AU - Wang, Shifei
AU - Maoka, Takashi
AU - Kawai-Noma, Shigeko
AU - Saito, Kyoichi
AU - Umeno, Daisuke
N1 - Funding Information:
This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology [JSPS KAKENHI Grants 15H04189, 15K14228, and 16H06450], the Hamaguchi Foundation for the Advancement of Biochemistry, the Futaba Electronics Memorial Foundation, and the Shorai Foundation for Science and Technology. L.L. is supported by a JSPS fellowship for young scientists [JSPS KAKENHI Grant 15J07486].
Publisher Copyright:
© 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - While the majority of the natural carotenoid pigments are based on 40-carbon (C 40 ) skeleton, some carotenoids from bacteria have larger C 50 skeleton, biosynthesized by attaching two isoprene units (C 5 ) to both sides of the C 40 carotenoid pigment lycopene. Subsequent cyclization reactions result in the production of C 50 carotenoids with diverse and unique skeletal structures. To produce even larger nonnatural novel carotenoids with C 50 + C 5 + C 5 = C 60 skeletons, we systematically coexpressed natural C 50 carotenoid biosynthetic enzymes (lycopene C 5 -elongases and C 50 -cyclases) from various bacterial sources together with the laboratory-engineered nonnatural C 50 -lycopene pathway in Escherichia coli. Among the tested enzymes, the elongases and cyclases from Micrococcus luteus exhibited significant activity toward C 50 -lycopene, and yielded the novel carotenoids C 60 -flavuxanthin and C 60 -sarcinaxanthin. Moreover, coexpression of M. luteus elongase with Corynebacterium cyclase resulted in the production of C 60 -sarcinaxanthin, C 60 -sarprenoxanthin, and C 60 -decaprenoxanthin.
AB - While the majority of the natural carotenoid pigments are based on 40-carbon (C 40 ) skeleton, some carotenoids from bacteria have larger C 50 skeleton, biosynthesized by attaching two isoprene units (C 5 ) to both sides of the C 40 carotenoid pigment lycopene. Subsequent cyclization reactions result in the production of C 50 carotenoids with diverse and unique skeletal structures. To produce even larger nonnatural novel carotenoids with C 50 + C 5 + C 5 = C 60 skeletons, we systematically coexpressed natural C 50 carotenoid biosynthetic enzymes (lycopene C 5 -elongases and C 50 -cyclases) from various bacterial sources together with the laboratory-engineered nonnatural C 50 -lycopene pathway in Escherichia coli. Among the tested enzymes, the elongases and cyclases from Micrococcus luteus exhibited significant activity toward C 50 -lycopene, and yielded the novel carotenoids C 60 -flavuxanthin and C 60 -sarcinaxanthin. Moreover, coexpression of M. luteus elongase with Corynebacterium cyclase resulted in the production of C 60 -sarcinaxanthin, C 60 -sarprenoxanthin, and C 60 -decaprenoxanthin.
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U2 - 10.1038/s41598-019-39289-w
DO - 10.1038/s41598-019-39289-w
M3 - Article
C2 - 30814614
AN - SCOPUS:85062233897
SN - 2045-2322
VL - 9
JO - Scientific reports
JF - Scientific reports
IS - 1
M1 - 2982
ER -