TY - JOUR
T1 - Hemostatic effects of polymerized albumin particles bearing rGPIa/IIa in thrombocytopenic mice
AU - Teramura, Yuji
AU - Okamura, Yosuke
AU - Takeoka, Shinji
AU - Tsuchiyama, Hiromi
AU - Narumi, Hideki
AU - Kainoh, Mie
AU - Handa, Makoto
AU - Ikeda, Yasuo
AU - Tsuchida, Eishun
N1 - Funding Information:
The authors thank Mr. S. Hara at Toray Industries, Inc., for in vivo evaluation of rGPIa/IIa–polyAlb. This work was supported by Health and Labour Sciences Research Grants, Research on Pharmaceutical and Medical Safety, Ministry of Health, Labor and Welfare, Japan, Grants-in-Aid (No. 11877172), and 21COE “Practical Nano-Chemistry” from MEXT, Japan. One of the authors (Y.T.) thanks Research Fellowships of the Japan Society for the Promotion of Science for Young Scientists.
PY - 2003/6/20
Y1 - 2003/6/20
N2 - The recombinant fragment of the platelet membrane glycoprotein Ia/IIa (rGPIa/IIa) was conjugated to the polymerized albumin particles (polyAlb) with the average diameter of 180nm. The intravenous administration of rGPIa/IIa-polyAlb to thrombocytopenic mice ([platelet]=2.1±0.3×105particles/μL) with three doses of ca. 2.4×1010, 7.2×1010, and 2.4×1011particles/kg, respectively, significantly reduced their bleeding time to 426±71, 378±101, and 337±46s, respectively, whereas that of the control groups (PBS) was 730±198s. The injection of rGPIa/IIa-polyAlb (2.4×1011particles/kg) was approximately equal to the effect of the injection of the mouse platelets at a dose of 2.0×1010 particles/kg. It was confirmed that rGPIa/IIa-polyAlb had a recognition ability against collagen and could contribute to the hemostasis in the thrombocytopenic mice as a platelet substitute.
AB - The recombinant fragment of the platelet membrane glycoprotein Ia/IIa (rGPIa/IIa) was conjugated to the polymerized albumin particles (polyAlb) with the average diameter of 180nm. The intravenous administration of rGPIa/IIa-polyAlb to thrombocytopenic mice ([platelet]=2.1±0.3×105particles/μL) with three doses of ca. 2.4×1010, 7.2×1010, and 2.4×1011particles/kg, respectively, significantly reduced their bleeding time to 426±71, 378±101, and 337±46s, respectively, whereas that of the control groups (PBS) was 730±198s. The injection of rGPIa/IIa-polyAlb (2.4×1011particles/kg) was approximately equal to the effect of the injection of the mouse platelets at a dose of 2.0×1010 particles/kg. It was confirmed that rGPIa/IIa-polyAlb had a recognition ability against collagen and could contribute to the hemostasis in the thrombocytopenic mice as a platelet substitute.
KW - Bleeding time
KW - Collagen
KW - GPIa/IIa
KW - Platelet substitutes
KW - Platelets
KW - Polymerized albumin particles
KW - Thrombocytopenic mice
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U2 - 10.1016/S0006-291X(03)00943-4
DO - 10.1016/S0006-291X(03)00943-4
M3 - Article
C2 - 12788097
AN - SCOPUS:0038730717
SN - 0006-291X
VL - 306
SP - 256
EP - 260
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -