High altitude training increases reactive carbonyl derivatives but not lipid peroxidation in skeletal muscle of rats

Zsolt Radák*, Katsumi Asano, Ki Chul Lee, Hideki Ohno, Akihiro Nakamura, Hideko Nakamoto, Sataro Goto

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

79 Citations (Scopus)


The oxidative stress related consequences of physical training at high altitude are not known. The hypothesis was tested that physical training and exposure to high altitude have adverse effects on free radical generation and activities of antioxidant enzymes. The present results showed that 4 weeks of exercise at an altitude of 4000 m increased the activity of Mn-SOD in both white and red types of skeletal muscle. The activities of Cu,Zn-SOD, catalase, and glutathione peroxidase, as well as the level of lipid peroxidation measured by TBARS and lipid hydroperoxides, did not change significantly. In contrast, the level of reactive carbonyl derivatives measured by anti-2,4-dinitrophenylhydrazone antibodies and spectrophotometry showed an increase in both types of muscle of altitude trained rats compared with sea level trained and control groups. It was suggested that the oxidative modification of certain amino acids is due to the increasing gap between activity of SOD and peroxide scavenging enzymes, which results in increases in the number of hydrogen peroxide molecules. Thus, since the mechanism of generation and/or the mode of action of radicals resulting in lipid peroxidation and protein oxidation appears to be different in vivo, both processes should be studied during oxidative stress.

Original languageEnglish
Pages (from-to)1109-1114
Number of pages6
JournalFree Radical Biology and Medicine
Issue number6
Publication statusPublished - 1997
Externally publishedYes


  • Antioxidant enzymes
  • Exercise
  • Free radical
  • High altitude
  • Lipid peroxidation
  • Reactive carbonyl derivatives

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)


Dive into the research topics of 'High altitude training increases reactive carbonyl derivatives but not lipid peroxidation in skeletal muscle of rats'. Together they form a unique fingerprint.

Cite this