High performance electrophoretic analysis on cycloolefin polymer microchips

Fumihiko Kitagawa; Takafumi Suzuki; Jun Mizuno; Shuichi Shoji; Koji Otsuka

High performance electrophoretic analysis on cycloolefin polymer microchips

Fumihiko Kitagawa, Takafumi Suzuki, Jun Mizuno, Shuichi Shoji, Koji Otsuka

Research output: Contribution to conference › Paper › peer-review

Abstract

This paper reports an application of cycloolefin polymer (ZEONEX) for affinity microchip electrophoresis (AMCE), and an improvement of the detectability for chiral analyses by using a thermal lens microscope (TLM) as a detection scheme and a sweeping technique based on a strong affinity between amino acids and proteins. In the chiral analysis of racemic tryptophan derivatized with a dye on the ZEONEX chip, the affinity sweeping by bovine serum albumin provided a 26-fold sensitivity enhancement compared to a conventional AMCE analysis.

Original language	English
Pages	362-364
Number of pages	3
Publication status	Published - 2006 Jan 1
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 2006 Nov 5 → 2006 Nov 9

Conference

Conference	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	06/11/5 → 06/11/9

Keywords

Chiral separation
Microchip electrophoresis
Protein
Sweeping
Thermal lens microscope

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

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title = "High performance electrophoretic analysis on cycloolefin polymer microchips",

abstract = "This paper reports an application of cycloolefin polymer (ZEONEX) for affinity microchip electrophoresis (AMCE), and an improvement of the detectability for chiral analyses by using a thermal lens microscope (TLM) as a detection scheme and a sweeping technique based on a strong affinity between amino acids and proteins. In the chiral analysis of racemic tryptophan derivatized with a dye on the ZEONEX chip, the affinity sweeping by bovine serum albumin provided a 26-fold sensitivity enhancement compared to a conventional AMCE analysis.",

keywords = "Chiral separation, Microchip electrophoresis, Protein, Sweeping, Thermal lens microscope",

author = "Fumihiko Kitagawa and Takafumi Suzuki and Jun Mizuno and Shuichi Shoji and Koji Otsuka",

year = "2006",

month = jan,

day = "1",

language = "English",

pages = "362--364",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

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TY - CONF

T1 - High performance electrophoretic analysis on cycloolefin polymer microchips

AU - Kitagawa, Fumihiko

AU - Suzuki, Takafumi

AU - Mizuno, Jun

AU - Shoji, Shuichi

AU - Otsuka, Koji

PY - 2006/1/1

Y1 - 2006/1/1

N2 - This paper reports an application of cycloolefin polymer (ZEONEX) for affinity microchip electrophoresis (AMCE), and an improvement of the detectability for chiral analyses by using a thermal lens microscope (TLM) as a detection scheme and a sweeping technique based on a strong affinity between amino acids and proteins. In the chiral analysis of racemic tryptophan derivatized with a dye on the ZEONEX chip, the affinity sweeping by bovine serum albumin provided a 26-fold sensitivity enhancement compared to a conventional AMCE analysis.

AB - This paper reports an application of cycloolefin polymer (ZEONEX) for affinity microchip electrophoresis (AMCE), and an improvement of the detectability for chiral analyses by using a thermal lens microscope (TLM) as a detection scheme and a sweeping technique based on a strong affinity between amino acids and proteins. In the chiral analysis of racemic tryptophan derivatized with a dye on the ZEONEX chip, the affinity sweeping by bovine serum albumin provided a 26-fold sensitivity enhancement compared to a conventional AMCE analysis.

KW - Chiral separation

KW - Microchip electrophoresis

KW - Protein

KW - Sweeping

KW - Thermal lens microscope

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M3 - Paper

AN - SCOPUS:84902523691

SP - 362

EP - 364

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

High performance electrophoretic analysis on cycloolefin polymer microchips

Abstract

Conference

Keywords

ASJC Scopus subject areas

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