High performance microfluidic device for sequential trapping, labeling and content extraction of single cells

Yoichi Murakami; Takahiro Arakawa; Sachiko Nomura; Yoshinori Yamaguchi; Shuichi Shoji

High performance microfluidic device for sequential trapping, labeling and content extraction of single cells

Yoichi Murakami, Takahiro Arakawa, Sachiko Nomura, Yoshinori Yamaguchi, Shuichi Shoji

School of Fundamental Science and Engineering

Research output: Contribution to conference › Paper › peer-review

3 Citations (Scopus)

Abstract

In this paper, we developed a high performance PDMS microfluidic device for sequential trapping, labeling and content extraction from single cells. The high performance single cell manipulation device consists of a main channel, single cell capture pockets, drain channels, and single cell lysate collection channels. The spontaneous single cell lysate extraction in the single cell pocket with chemical solution of 10% sodium dodecyl sulfate (SDS) was achieved. Since this device demonstrated the successive single cell isolation, labeling and single-cell lysate extraction, the pretreatment of the capillary electrophoresis can be completed.

Original language	English
Pages	1774-1776
Number of pages	3
Publication status	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 2008 Oct 12 → 2008 Oct 16

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	08/10/12 → 08/10/16

Keywords

Cell labeling
Cell manipulation
Cell trapping
Single cell lysate extraction

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

High performance microfluidic device for sequential trapping, labeling and content extraction of single cells. / Murakami, Yoichi; Arakawa, Takahiro; Nomura, Sachiko et al.
2008. 1774-1776 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

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abstract = "In this paper, we developed a high performance PDMS microfluidic device for sequential trapping, labeling and content extraction from single cells. The high performance single cell manipulation device consists of a main channel, single cell capture pockets, drain channels, and single cell lysate collection channels. The spontaneous single cell lysate extraction in the single cell pocket with chemical solution of 10% sodium dodecyl sulfate (SDS) was achieved. Since this device demonstrated the successive single cell isolation, labeling and single-cell lysate extraction, the pretreatment of the capillary electrophoresis can be completed.",

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T1 - High performance microfluidic device for sequential trapping, labeling and content extraction of single cells

AU - Murakami, Yoichi

AU - Arakawa, Takahiro

AU - Nomura, Sachiko

AU - Yamaguchi, Yoshinori

AU - Shoji, Shuichi

PY - 2008

Y1 - 2008

N2 - In this paper, we developed a high performance PDMS microfluidic device for sequential trapping, labeling and content extraction from single cells. The high performance single cell manipulation device consists of a main channel, single cell capture pockets, drain channels, and single cell lysate collection channels. The spontaneous single cell lysate extraction in the single cell pocket with chemical solution of 10% sodium dodecyl sulfate (SDS) was achieved. Since this device demonstrated the successive single cell isolation, labeling and single-cell lysate extraction, the pretreatment of the capillary electrophoresis can be completed.

AB - In this paper, we developed a high performance PDMS microfluidic device for sequential trapping, labeling and content extraction from single cells. The high performance single cell manipulation device consists of a main channel, single cell capture pockets, drain channels, and single cell lysate collection channels. The spontaneous single cell lysate extraction in the single cell pocket with chemical solution of 10% sodium dodecyl sulfate (SDS) was achieved. Since this device demonstrated the successive single cell isolation, labeling and single-cell lysate extraction, the pretreatment of the capillary electrophoresis can be completed.

KW - Cell labeling

KW - Cell manipulation

KW - Cell trapping

KW - Single cell lysate extraction

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M3 - Paper

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SP - 1774

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T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

High performance microfluidic device for sequential trapping, labeling and content extraction of single cells

Abstract

Conference

Keywords

ASJC Scopus subject areas

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