High performance parallel bioparticle sorter with 3-dimensional PDMS chip

H. Sugino; Y. Nara; Y. Shirasaki; T. Arakawa; S. Shoji; T. Funatsu

High performance parallel bioparticle sorter with 3-dimensional PDMS chip

H. Sugino, Y. Nara, Y. Shirasaki, T. Arakawa, S. Shoji, T. Funatsu

School of Fundamental Science and Engineering

Research output: Contribution to conference › Paper › peer-review

Abstract

This paper reports an improved method for achieving on-chip parallel sorting of fluorescently labeled bioparticles. We have integrated 8-parallel sheath flow sorters, in which a sample stream is sheathed by two buffer streams to focus the sample stream, into a single chip. Each sorter integrated in the chip successfully sorted fluorescent microspheres and Escherichia coli (E. coli) cells expressing fluorescent proteins with 10-20 ms of flow switching time and an 85-95% recovery ratio.

Original language	English
Pages	1184-1186
Number of pages	3
Publication status	Published - 2008 Jan 1
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 2008 Oct 12 → 2008 Oct 16

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	08/10/12 → 08/10/16

Keywords

Microfluidics
Multi-layer PDMS
Parallel sorting
Sol-gel transition

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

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title = "High performance parallel bioparticle sorter with 3-dimensional PDMS chip",

abstract = "This paper reports an improved method for achieving on-chip parallel sorting of fluorescently labeled bioparticles. We have integrated 8-parallel sheath flow sorters, in which a sample stream is sheathed by two buffer streams to focus the sample stream, into a single chip. Each sorter integrated in the chip successfully sorted fluorescent microspheres and Escherichia coli (E. coli) cells expressing fluorescent proteins with 10-20 ms of flow switching time and an 85-95% recovery ratio.",

keywords = "Microfluidics, Multi-layer PDMS, Parallel sorting, Sol-gel transition",

author = "H. Sugino and Y. Nara and Y. Shirasaki and T. Arakawa and S. Shoji and T. Funatsu",

year = "2008",

month = jan,

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T1 - High performance parallel bioparticle sorter with 3-dimensional PDMS chip

AU - Sugino, H.

AU - Nara, Y.

AU - Shirasaki, Y.

AU - Arakawa, T.

AU - Shoji, S.

AU - Funatsu, T.

PY - 2008/1/1

Y1 - 2008/1/1

N2 - This paper reports an improved method for achieving on-chip parallel sorting of fluorescently labeled bioparticles. We have integrated 8-parallel sheath flow sorters, in which a sample stream is sheathed by two buffer streams to focus the sample stream, into a single chip. Each sorter integrated in the chip successfully sorted fluorescent microspheres and Escherichia coli (E. coli) cells expressing fluorescent proteins with 10-20 ms of flow switching time and an 85-95% recovery ratio.

AB - This paper reports an improved method for achieving on-chip parallel sorting of fluorescently labeled bioparticles. We have integrated 8-parallel sheath flow sorters, in which a sample stream is sheathed by two buffer streams to focus the sample stream, into a single chip. Each sorter integrated in the chip successfully sorted fluorescent microspheres and Escherichia coli (E. coli) cells expressing fluorescent proteins with 10-20 ms of flow switching time and an 85-95% recovery ratio.

KW - Microfluidics

KW - Multi-layer PDMS

KW - Parallel sorting

KW - Sol-gel transition

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M3 - Paper

AN - SCOPUS:84902438971

SP - 1184

EP - 1186

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

High performance parallel bioparticle sorter with 3-dimensional PDMS chip

Abstract

Conference

Keywords

ASJC Scopus subject areas

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