High-throughput single-cell secretion measurement on an optical wavegude chip

Y. Shirasaki; N. Suzuki; M. Yamagishi; A. Nakahara; Shuichi Shoji; O. Ohara

High-throughput single-cell secretion measurement on an optical wavegude chip

Y. Shirasaki^*, N. Suzuki, M. Yamagishi, A. Nakahara, Shuichi Shoji, O. Ohara

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

We report a newly developed platform that enables us to monitor protein secretion from more than hundreds individual single cells in real time simultaneously. The platform was achieved after improvement of the limited size of view field in total internal reflection fluorescence microscopy, which was owing to using optical waveguide technology. Although the coupling of PDMS microwells and glass waveguides raised another problem regarding elevated background, it was successfully solved by sandwiched a thin layer of the amorphous fluoropolymer between the glass waveguide and the PDMS microwell. Protein secretion from about 900 cells was successfully observed on the platform.

Original language	English
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Publisher	Chemical and Biological Microsystems Society
Pages	831-833
Number of pages	3
ISBN (Print)	9780979806476
Publication status	Published - 2014
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States Duration: 2014 Oct 26 → 2014 Oct 30

Other

Other	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Country/Territory	United States
City	San Antonio
Period	14/10/26 → 14/10/30

Keywords

Secretion measurement
Single-cell
TIRFM

ASJC Scopus subject areas

Control and Systems Engineering

Cite this

High-throughput single-cell secretion measurement on an optical wavegude chip. / Shirasaki, Y.; Suzuki, N.; Yamagishi, M. et al.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 831-833.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Shirasaki, Y, Suzuki, N, Yamagishi, M, Nakahara, A, Shoji, S & Ohara, O 2014, High-throughput single-cell secretion measurement on an optical wavegude chip. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, pp. 831-833, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 14/10/26.

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title = "High-throughput single-cell secretion measurement on an optical wavegude chip",

abstract = "We report a newly developed platform that enables us to monitor protein secretion from more than hundreds individual single cells in real time simultaneously. The platform was achieved after improvement of the limited size of view field in total internal reflection fluorescence microscopy, which was owing to using optical waveguide technology. Although the coupling of PDMS microwells and glass waveguides raised another problem regarding elevated background, it was successfully solved by sandwiched a thin layer of the amorphous fluoropolymer between the glass waveguide and the PDMS microwell. Protein secretion from about 900 cells was successfully observed on the platform.",

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author = "Y. Shirasaki and N. Suzuki and M. Yamagishi and A. Nakahara and Shuichi Shoji and O. Ohara",

year = "2014",

language = "English",

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pages = "831--833",

booktitle = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

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AU - Shirasaki, Y.

AU - Suzuki, N.

AU - Yamagishi, M.

AU - Nakahara, A.

AU - Shoji, Shuichi

AU - Ohara, O.

PY - 2014

Y1 - 2014

N2 - We report a newly developed platform that enables us to monitor protein secretion from more than hundreds individual single cells in real time simultaneously. The platform was achieved after improvement of the limited size of view field in total internal reflection fluorescence microscopy, which was owing to using optical waveguide technology. Although the coupling of PDMS microwells and glass waveguides raised another problem regarding elevated background, it was successfully solved by sandwiched a thin layer of the amorphous fluoropolymer between the glass waveguide and the PDMS microwell. Protein secretion from about 900 cells was successfully observed on the platform.

AB - We report a newly developed platform that enables us to monitor protein secretion from more than hundreds individual single cells in real time simultaneously. The platform was achieved after improvement of the limited size of view field in total internal reflection fluorescence microscopy, which was owing to using optical waveguide technology. Although the coupling of PDMS microwells and glass waveguides raised another problem regarding elevated background, it was successfully solved by sandwiched a thin layer of the amorphous fluoropolymer between the glass waveguide and the PDMS microwell. Protein secretion from about 900 cells was successfully observed on the platform.

KW - Secretion measurement

KW - Single-cell

KW - TIRFM

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M3 - Conference contribution

AN - SCOPUS:84941671387

SN - 9780979806476

SP - 831

EP - 833

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biological Microsystems Society

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Y2 - 26 October 2014 through 30 October 2014

ER -

High-throughput single-cell secretion measurement on an optical wavegude chip

Abstract

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Keywords

ASJC Scopus subject areas

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