TY - JOUR
T1 - High-throughput SNP detection using nano-scale engineered biomagnetite
AU - Matsunaga, Tadashi
AU - Maruyama, Kohei
AU - Takeyama, Haruko
AU - Katoh, Takahiko
N1 - Funding Information:
This work was funded in part by a Grant-in-Aid for Specially Promoted Research, No. 13002005 and Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan. It was also supported by NEDO's Subsidy for FY2002 University-initiated Practical Business Creation R&D Program.
PY - 2007/4/15
Y1 - 2007/4/15
N2 - A semi-automated system for the large-scale detection of single nucleotide polymorphisms (SNPs) has been developed based on allele-specific oligonucleotide hybridization and thermal dissociation curve analysis using nano-scale engineered biomagnetite (bacterial magnetic particles; BacMPs). For reliable detection in large numbers of samples, several conditions for the capture of target DNA on nano-sized BacMPs and the denaturation of double-stranded DNA were optimized. The most efficient target DNA capture was observed using short PCR amplicons (69 bp). Captured DNAs were denatured using 50 mM NaOH. With these optimizations, large-scale SNP detection was performed on 822 samples of the transforming growth factor (TGF)-β1 gene, which is rich in both GC content and repetitive sequences. High reliability for the semi-automated BacMP-based SNP detection system was confirmed following comparison to traditional sequencing-based methods.
AB - A semi-automated system for the large-scale detection of single nucleotide polymorphisms (SNPs) has been developed based on allele-specific oligonucleotide hybridization and thermal dissociation curve analysis using nano-scale engineered biomagnetite (bacterial magnetic particles; BacMPs). For reliable detection in large numbers of samples, several conditions for the capture of target DNA on nano-sized BacMPs and the denaturation of double-stranded DNA were optimized. The most efficient target DNA capture was observed using short PCR amplicons (69 bp). Captured DNAs were denatured using 50 mM NaOH. With these optimizations, large-scale SNP detection was performed on 822 samples of the transforming growth factor (TGF)-β1 gene, which is rich in both GC content and repetitive sequences. High reliability for the semi-automated BacMP-based SNP detection system was confirmed following comparison to traditional sequencing-based methods.
KW - Automated system
KW - Bacterial magnetic particles (BacMPs)
KW - Hybridization
KW - Nano-scale engineered biomagnetite
KW - Single nucleotide polymorphism (SNP)
KW - Transforming growth factor (TGF)-β1
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U2 - 10.1016/j.bios.2006.12.022
DO - 10.1016/j.bios.2006.12.022
M3 - Article
C2 - 17270414
AN - SCOPUS:33847349678
SN - 0956-5663
VL - 22
SP - 2315
EP - 2321
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
IS - 9-10
ER -