Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice

Yasunori Sato; Shizuo Kajiyama; Akiko Amano; Yoshitaka Kondo; Toru Sasaki; Setsuko Handa; Ryoya Takahashi; Michiaki Fukui; Goji Hasegawa; Naoto Nakamura; Hikohito Fujinawa; Toyotaka Mori; Mitsuhiro Ohta; Hiroshi Obayashi; Naoki Maruyama; Akihito Ishigami

doi:10.1016/j.bbrc.2008.08.020

Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice

Yasunori Sato, Shizuo Kajiyama, Akiko Amano, Yoshitaka Kondo, Toru Sasaki, Setsuko Handa, Ryoya Takahashi, Michiaki Fukui, Goji Hasegawa, Naoto Nakamura, Hikohito Fujinawa, Toyotaka Mori, Mitsuhiro Ohta, Hiroshi Obayashi, Naoki Maruyama, Akihito Ishigami^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

135 Citations (Scopus)

Abstract

Hydrogen is an established anti-oxidant that prevents acute oxidative stress. To clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H₂) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), also a well-known anti-oxidant. These KO mice were divided into three groups; recipients of H₂, VC, or pure water (H₂O), administered for 33 days. VC levels in H₂ and H₂O groups were <6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H₂ group subjected to ischemia-reperfusion than in the H₂O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation.

Original language	English
Pages (from-to)	346-350
Number of pages	5
Journal	Biochemical and Biophysical Research Communications
Volume	375
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2008.08.020
Publication status	Published - 2008 Oct 24
Externally published	Yes

Keywords

Ascorbic acid
Chemiluminescence
Gluconolactonase
Hydrogen-rich pure water
Oxidative stress
ROS
Senescence marker protein-30
Superoxide
Vitamin C

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2008.08.020

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Sato, Y., Kajiyama, S., Amano, A., Kondo, Y., Sasaki, T., Handa, S., Takahashi, R., Fukui, M., Hasegawa, G., Nakamura, N., Fujinawa, H., Mori, T., Ohta, M., Obayashi, H., Maruyama, N., & Ishigami, A. (2008). Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice. Biochemical and Biophysical Research Communications, 375(3), 346-350. https://doi.org/10.1016/j.bbrc.2008.08.020

Sato, Y, Kajiyama, S, Amano, A, Kondo, Y, Sasaki, T, Handa, S, Takahashi, R, Fukui, M, Hasegawa, G, Nakamura, N, Fujinawa, H, Mori, T, Ohta, M, Obayashi, H, Maruyama, N & Ishigami, A 2008, 'Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice', Biochemical and Biophysical Research Communications, vol. 375, no. 3, pp. 346-350. https://doi.org/10.1016/j.bbrc.2008.08.020

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title = "Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice",

abstract = "Hydrogen is an established anti-oxidant that prevents acute oxidative stress. To clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H2) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), also a well-known anti-oxidant. These KO mice were divided into three groups; recipients of H2, VC, or pure water (H2O), administered for 33 days. VC levels in H2 and H2O groups were <6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H2 group subjected to ischemia-reperfusion than in the H2O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation.",

keywords = "Ascorbic acid, Chemiluminescence, Gluconolactonase, Hydrogen-rich pure water, Oxidative stress, ROS, Senescence marker protein-30, Superoxide, Vitamin C",

author = "Yasunori Sato and Shizuo Kajiyama and Akiko Amano and Yoshitaka Kondo and Toru Sasaki and Setsuko Handa and Ryoya Takahashi and Michiaki Fukui and Goji Hasegawa and Naoto Nakamura and Hikohito Fujinawa and Toyotaka Mori and Mitsuhiro Ohta and Hiroshi Obayashi and Naoki Maruyama and Akihito Ishigami",

note = "Funding Information: This study is supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture, Japan (to A.I., S.H. and N.S.), and a Grant-in-Aid for Smoking Research Foundation, Japan (to A.I.). We thank Ms. P. Minick for the excellent English editorial assistance. Vitamin C powder was kindly provided by DSM Nutrition Japan.",

year = "2008",

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doi = "10.1016/j.bbrc.2008.08.020",

language = "English",

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T1 - Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice

AU - Sato, Yasunori

AU - Kajiyama, Shizuo

AU - Amano, Akiko

AU - Kondo, Yoshitaka

AU - Sasaki, Toru

AU - Handa, Setsuko

AU - Takahashi, Ryoya

AU - Fukui, Michiaki

AU - Hasegawa, Goji

AU - Nakamura, Naoto

AU - Fujinawa, Hikohito

AU - Mori, Toyotaka

AU - Ohta, Mitsuhiro

AU - Obayashi, Hiroshi

AU - Maruyama, Naoki

AU - Ishigami, Akihito

N1 - Funding Information: This study is supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, and Culture, Japan (to A.I., S.H. and N.S.), and a Grant-in-Aid for Smoking Research Foundation, Japan (to A.I.). We thank Ms. P. Minick for the excellent English editorial assistance. Vitamin C powder was kindly provided by DSM Nutrition Japan.

PY - 2008/10/24

Y1 - 2008/10/24

N2 - Hydrogen is an established anti-oxidant that prevents acute oxidative stress. To clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H2) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), also a well-known anti-oxidant. These KO mice were divided into three groups; recipients of H2, VC, or pure water (H2O), administered for 33 days. VC levels in H2 and H2O groups were <6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H2 group subjected to ischemia-reperfusion than in the H2O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation.

AB - Hydrogen is an established anti-oxidant that prevents acute oxidative stress. To clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H2) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), also a well-known anti-oxidant. These KO mice were divided into three groups; recipients of H2, VC, or pure water (H2O), administered for 33 days. VC levels in H2 and H2O groups were <6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H2 group subjected to ischemia-reperfusion than in the H2O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation.

KW - Ascorbic acid

KW - Chemiluminescence

KW - Gluconolactonase

KW - Hydrogen-rich pure water

KW - Oxidative stress

KW - ROS

KW - Senescence marker protein-30

KW - Superoxide

KW - Vitamin C

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SN - 0006-291X

VL - 375

SP - 346

EP - 350

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice

Abstract

Keywords

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