Hydrogen-rich pure water prevents superoxide formation in brain slices of vitamin C-depleted SMP30/GNL knockout mice

Yasunori Sato, Shizuo Kajiyama, Akiko Amano, Yoshitaka Kondo, Toru Sasaki, Setsuko Handa, Ryoya Takahashi, Michiaki Fukui, Goji Hasegawa, Naoto Nakamura, Hikohito Fujinawa, Toyotaka Mori, Mitsuhiro Ohta, Hiroshi Obayashi, Naoki Maruyama, Akihito Ishigami*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

135 Citations (Scopus)


Hydrogen is an established anti-oxidant that prevents acute oxidative stress. To clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H2) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), also a well-known anti-oxidant. These KO mice were divided into three groups; recipients of H2, VC, or pure water (H2O), administered for 33 days. VC levels in H2 and H2O groups were <6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H2 group subjected to ischemia-reperfusion than in the H2O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation.

Original languageEnglish
Pages (from-to)346-350
Number of pages5
JournalBiochemical and Biophysical Research Communications
Issue number3
Publication statusPublished - 2008 Oct 24
Externally publishedYes


  • Ascorbic acid
  • Chemiluminescence
  • Gluconolactonase
  • Hydrogen-rich pure water
  • Oxidative stress
  • ROS
  • Senescence marker protein-30
  • Superoxide
  • Vitamin C

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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