Identification of membrane and secreted proteins in anterior cruciate ligament derived cells using "signal-sequence-trap", a retrovirus-mediated expression screening method

Shigeru Miyaki; Ken ich Tezuka; Takashi Ushida; Takayuki Akimoto; Hiroshi Akaogi; Toshio Kitamura; Yutaka Miyanaga; Junzo Tanaka; Naoyuki Ochiai; Tetsuya Tateishi

doi:10.1016/j.msec.2003.12.012

Identification of membrane and secreted proteins in anterior cruciate ligament derived cells using "signal-sequence-trap", a retrovirus-mediated expression screening method

Shigeru Miyaki^*, Ken ich Tezuka, Takashi Ushida, Takayuki Akimoto, Hiroshi Akaogi, Toshio Kitamura, Yutaka Miyanaga, Junzo Tanaka, Naoyuki Ochiai, Tetsuya Tateishi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Ligament injuries are one of most common injuries to the knee joint; however, little is known about how ligaments heal and what kinds of precursor cells participate in ligament repair. Ligament cells were thought to be members of one of the lineages from mesenchymal stem cells (MSCs). However, there have been no reports on the differentiation mechanism of MSC to ligament cells because they do not have known phenotypic markers. To identify the anterior cruciate ligament (ACL)-specific phenotypic marker, we screened a cDNA library derived from rabbit ACL cells, using the signal sequence trap by the retrovirus-mediated expression screening (SST-REX) method. We detected known clones representing 135 independent proteins. Among them, 120 were secreted proteins and 15 were membrane proteins. Some of the clones encoded proteins that have never been reported to be expressed in ACLs. Therefore, the data could provide a better understanding of ACL cells and could help in finding their phenotypic markers.

Original language	English
Pages (from-to)	397-401
Number of pages	5
Journal	Materials Science and Engineering C
Volume	24
Issue number	3
DOIs	https://doi.org/10.1016/j.msec.2003.12.012
Publication status	Published - 2004 Apr 1
Externally published	Yes

Keywords

Ligament
Proteins
Retrovirus

ASJC Scopus subject areas

Materials Science(all)
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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10.1016/j.msec.2003.12.012

Cite this

Miyaki, S., Tezuka, K. I., Ushida, T., Akimoto, T., Akaogi, H., Kitamura, T., Miyanaga, Y., Tanaka, J., Ochiai, N., & Tateishi, T. (2004). Identification of membrane and secreted proteins in anterior cruciate ligament derived cells using "signal-sequence-trap", a retrovirus-mediated expression screening method. Materials Science and Engineering C, 24(3), 397-401. https://doi.org/10.1016/j.msec.2003.12.012

Identification of membrane and secreted proteins in anterior cruciate ligament derived cells using "signal-sequence-trap", a retrovirus-mediated expression screening method. / Miyaki, Shigeru; Tezuka, Ken ich; Ushida, Takashi et al.
In: Materials Science and Engineering C, Vol. 24, No. 3, 01.04.2004, p. 397-401.

Research output: Contribution to journal › Article › peer-review

Miyaki, S, Tezuka, KI, Ushida, T, Akimoto, T, Akaogi, H, Kitamura, T, Miyanaga, Y, Tanaka, J, Ochiai, N & Tateishi, T 2004, 'Identification of membrane and secreted proteins in anterior cruciate ligament derived cells using "signal-sequence-trap", a retrovirus-mediated expression screening method', Materials Science and Engineering C, vol. 24, no. 3, pp. 397-401. https://doi.org/10.1016/j.msec.2003.12.012

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abstract = "Ligament injuries are one of most common injuries to the knee joint; however, little is known about how ligaments heal and what kinds of precursor cells participate in ligament repair. Ligament cells were thought to be members of one of the lineages from mesenchymal stem cells (MSCs). However, there have been no reports on the differentiation mechanism of MSC to ligament cells because they do not have known phenotypic markers. To identify the anterior cruciate ligament (ACL)-specific phenotypic marker, we screened a cDNA library derived from rabbit ACL cells, using the signal sequence trap by the retrovirus-mediated expression screening (SST-REX) method. We detected known clones representing 135 independent proteins. Among them, 120 were secreted proteins and 15 were membrane proteins. Some of the clones encoded proteins that have never been reported to be expressed in ACLs. Therefore, the data could provide a better understanding of ACL cells and could help in finding their phenotypic markers.",

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AU - Akimoto, Takayuki

AU - Akaogi, Hiroshi

AU - Kitamura, Toshio

AU - Miyanaga, Yutaka

AU - Tanaka, Junzo

AU - Ochiai, Naoyuki

AU - Tateishi, Tetsuya

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PY - 2004/4/1

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N2 - Ligament injuries are one of most common injuries to the knee joint; however, little is known about how ligaments heal and what kinds of precursor cells participate in ligament repair. Ligament cells were thought to be members of one of the lineages from mesenchymal stem cells (MSCs). However, there have been no reports on the differentiation mechanism of MSC to ligament cells because they do not have known phenotypic markers. To identify the anterior cruciate ligament (ACL)-specific phenotypic marker, we screened a cDNA library derived from rabbit ACL cells, using the signal sequence trap by the retrovirus-mediated expression screening (SST-REX) method. We detected known clones representing 135 independent proteins. Among them, 120 were secreted proteins and 15 were membrane proteins. Some of the clones encoded proteins that have never been reported to be expressed in ACLs. Therefore, the data could provide a better understanding of ACL cells and could help in finding their phenotypic markers.

AB - Ligament injuries are one of most common injuries to the knee joint; however, little is known about how ligaments heal and what kinds of precursor cells participate in ligament repair. Ligament cells were thought to be members of one of the lineages from mesenchymal stem cells (MSCs). However, there have been no reports on the differentiation mechanism of MSC to ligament cells because they do not have known phenotypic markers. To identify the anterior cruciate ligament (ACL)-specific phenotypic marker, we screened a cDNA library derived from rabbit ACL cells, using the signal sequence trap by the retrovirus-mediated expression screening (SST-REX) method. We detected known clones representing 135 independent proteins. Among them, 120 were secreted proteins and 15 were membrane proteins. Some of the clones encoded proteins that have never been reported to be expressed in ACLs. Therefore, the data could provide a better understanding of ACL cells and could help in finding their phenotypic markers.

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Identification of membrane and secreted proteins in anterior cruciate ligament derived cells using "signal-sequence-trap", a retrovirus-mediated expression screening method

Abstract

Keywords

ASJC Scopus subject areas

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