Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain

Motoyuki Shimizu; Keiichi Higuchi; Beth Bennett; Chen Xia; Tadao Tsuboyama; Soichiro Kasai; Takuya Chiba; Hiromi Fujisawa; Kumiko Kogishi; Haruo Kitado; Mitsutoshi Kimoto; Norikazu Takeda; Mutsumi Matsushita; Hideo Okumura; Tadao Serikawa; Takashi Nakamura; Thomas E. Johnson; Masanori Hosokawa

doi:10.1007/s003359900949

Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain

Motoyuki Shimizu, Keiichi Higuchi, Beth Bennett, Chen Xia, Tadao Tsuboyama, Soichiro Kasai, Takuya Chiba, Hiromi Fujisawa, Kumiko Kogishi, Haruo Kitado, Mitsutoshi Kimoto, Norikazu Takeda, Mutsumi Matsushita, Hideo Okumura, Tadao Serikawa, Takashi Nakamura, Thomas E. Johnson, Masanori Hosokawa^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

94 Citations (Scopus)

Abstract

The whole genome scan for quantitative trait loci (QTLs) specifying peak bone mass was performed with the F₂ intercrosses of SAMP6, an established murine model of senile osteoporosis, exhibiting a significantly lower peak bone mass, and SAMP2, exhibiting a higher peak bone mass. Cortical thickness index (CTI), a parameter of bone mass of femurs, was measured in 488 F₂ progeny at 4 months of age, when the animals attained peak bone mass by microphotodensitometry. Genetic markers were typed at 90 loci spanning all chromosomes except the Y. By interval mapping of 246 male F₂ mice, two loci were identified with significant linkage to peak bone mass, one on Chromosome (Chr) 11 and another on Chr 13, with a maximum lod score of 10.8 (22.2% of the total variance) and 5.8 (10.0%), respectively. Another locus on the X Chr was suggestive of a QTL associated oppositely with a low peak bone mass to the SAMP2 allele. This association was consistent with the distribution of peak bone mass in the F₁ and F₂. These findings should be useful to elucidate the genetics of osteoporosis.

Original language	English
Pages (from-to)	81-87
Number of pages	7
Journal	Mammalian Genome
Volume	10
Issue number	2
DOIs	https://doi.org/10.1007/s003359900949
Publication status	Published - 1999
Externally published	Yes

ASJC Scopus subject areas

Genetics

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Shimizu, M., Higuchi, K., Bennett, B., Xia, C., Tsuboyama, T., Kasai, S., Chiba, T., Fujisawa, H., Kogishi, K., Kitado, H., Kimoto, M., Takeda, N., Matsushita, M., Okumura, H., Serikawa, T., Nakamura, T., Johnson, T. E., & Hosokawa, M. (1999). Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain. Mammalian Genome, 10(2), 81-87. https://doi.org/10.1007/s003359900949

Shimizu, M, Higuchi, K, Bennett, B, Xia, C, Tsuboyama, T, Kasai, S, Chiba, T, Fujisawa, H, Kogishi, K, Kitado, H, Kimoto, M, Takeda, N, Matsushita, M, Okumura, H, Serikawa, T, Nakamura, T, Johnson, TE & Hosokawa, M 1999, 'Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain', Mammalian Genome, vol. 10, no. 2, pp. 81-87. https://doi.org/10.1007/s003359900949

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abstract = "The whole genome scan for quantitative trait loci (QTLs) specifying peak bone mass was performed with the F2 intercrosses of SAMP6, an established murine model of senile osteoporosis, exhibiting a significantly lower peak bone mass, and SAMP2, exhibiting a higher peak bone mass. Cortical thickness index (CTI), a parameter of bone mass of femurs, was measured in 488 F2 progeny at 4 months of age, when the animals attained peak bone mass by microphotodensitometry. Genetic markers were typed at 90 loci spanning all chromosomes except the Y. By interval mapping of 246 male F2 mice, two loci were identified with significant linkage to peak bone mass, one on Chromosome (Chr) 11 and another on Chr 13, with a maximum lod score of 10.8 (22.2% of the total variance) and 5.8 (10.0%), respectively. Another locus on the X Chr was suggestive of a QTL associated oppositely with a low peak bone mass to the SAMP2 allele. This association was consistent with the distribution of peak bone mass in the F1 and F2. These findings should be useful to elucidate the genetics of osteoporosis.",

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AU - Higuchi, Keiichi

AU - Bennett, Beth

AU - Xia, Chen

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AU - Kasai, Soichiro

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AU - Fujisawa, Hiromi

AU - Kogishi, Kumiko

AU - Kitado, Haruo

AU - Kimoto, Mitsutoshi

AU - Takeda, Norikazu

AU - Matsushita, Mutsumi

AU - Okumura, Hideo

AU - Serikawa, Tadao

AU - Nakamura, Takashi

AU - Johnson, Thomas E.

AU - Hosokawa, Masanori

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Identification of peak bone mass QTL in a spontaneously osteoporotic mouse strain

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