@article{9c8df5b724e246adaa6faf3307d06149,
title = "Identification of Qk as a Glial Precursor Cell Marker that Governs the Fate Specification of Neural Stem Cells to a Glial Cell Lineage",
abstract = "During brain development, neural stem cells (NSCs) initially produce neurons and change their fate to generate glias. While the regulation of neurogenesis is well characterized, specific markers for glial precursor cells (GPCs) and the master regulators for gliogenesis remain unidentified. Accumulating evidence suggests that RNA-binding proteins (RBPs) have significant roles in neuronal development and function, as they comprehensively regulate the expression of target genes in a cell-type-specific manner. We systematically investigated the expression profiles of 1,436 murine RBPs in the developing mouse brain and identified quaking (Qk) as a marker of the putative GPC population. Functional analysis of the NSC-specific Qk-null mutant mouse revealed the key role of Qk in astrocyte and oligodendrocyte generation and differentiation from NSCs. Mechanistically, Qk upregulates gliogenic genes via quaking response elements in their 3′ untranslated regions. These results provide crucial directions for identifying GPCs and deciphering the regulatory mechanisms of gliogenesis from NSCs.",
keywords = "3′ untranslated region, RNA-binding protein, RNA-seq, endocytosis, glial precursor cells/GPCs, gliogenesis, mRNA stabilization, neural stem cells/NSCs, quaking/Qk, regulon",
author = "Akihide Takeuchi and Yuji Takahashi and Kei Iida and Motoyasu Hosokawa and Koichiro Irie and Mikako Ito and Brown, {J. B.} and Kinji Ohno and Kinichi Nakashima and Masatoshi Hagiwara",
note = "Funding Information: We thank the Kyoto University Medical Research Support Center for performing the high-throughput sequencing analysis and its Institute of Laboratory Animals for providing animal care; A. Hagiwara, A. Hirose, and K. Makigaya for technical assistance; Dr. T. Awaya for critical reading of the manuscript and helpful comments; and Editage (www.editage.jp) for English language editing of this article. This work was supported in part by the Japan Society for the Promotion of Science (JSPS) Grants-in-Aid for Scientific Research (KAKENHI) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT, JSPS KAKENHI 19500269, 25500288, 21249013, 15H05721, 19K06907) (to M. Hagiwara and A.T.); Innovative Cell Biology by Innovating Technology (Cell Innovation) (to M. Hagiwara, K.O. and A.T.); a Core Research for Evolutional Science and Technology grant from the Japan Science and Technology Agency (to M. Hagiwara); a grant from the Japan Agency for Medical Research and Development (to M. Hagiwara); the Asian CORE Program of JSPS (to M. Hagiwara); iCeMS Cross-Disciplinary Research Promotion Project of Kyoto University (to A.T.); and the Fujiwara Memorial Foundation (to A.T.). Funding Information: We thank the Kyoto University Medical Research Support Center for performing the high-throughput sequencing analysis and its Institute of Laboratory Animals for providing animal care; A. Hagiwara, A. Hirose, and K. Makigaya for technical assistance; Dr. T. Awaya for critical reading of the manuscript and helpful comments; and Editage ( www.editage.jp ) for English language editing of this article. This work was supported in part by the Japan Society for the Promotion of Science (JSPS) Grants-in-Aid for Scientific Research (KAKENHI) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT, JSPS KAKENHI 19500269 , 25500288 , 21249013 , 15H05721 , 19K06907 ) (to M. Hagiwara and A.T.); Innovative Cell Biology by Innovating Technology (Cell Innovation) (to M. Hagiwara, K.O., and A.T.); a Core Research for Evolutional Science and Technology grant from the Japan Science and Technology Agency (to M. Hagiwara); a grant from the Japan Agency for Medical Research and Development (to M. Hagiwara); the Asian CORE Program of JSPS (to M. Hagiwara); iCeMS Cross-Disciplinary Research Promotion Project of Kyoto University (to A.T.); and the Fujiwara Memorial Foundation (to A.T.). Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2020",
month = oct,
day = "13",
doi = "10.1016/j.stemcr.2020.08.010",
language = "English",
volume = "15",
pages = "883--897",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "4",
}